Manager of Next Generation Sequencing

Mount Sinai Genetic Testing Lab - Connecticut
Job Location
Branford, CT 06405
Job Description

SUMMARY: Mount Sinai is seeking a motivated Manager to join our CT sequencing team in a high complexity laboratory testing facility. With minimal supervision, the Sequencing Manager will oversee a team of Associate Researchers as they construct NGS libraries and operate a fleet of sequencers using automated methods. The Sequencing Manager will also draft SOPs, contribute to and execute assay validation test plans, drive technology development, and set the production schedule in a CLIA-certified diagnostic laboratory.

Primary Duties and Responsibilities:

* Oversee and contribute to generation of large, multiplexed pools of amplicons and

libraries from isolated gDNA using automated methods in a diagnostic laboratory


* Ensure accurate and timely entry of sample information into Laboratory Information

Management System (LIMS).

* Oversee and develop library construction and Next Generation Sequencing protocols

using automated methods and perform Next Generation Sequencing (NGS).

* Using established Standard Operating Procedures (SOPs), oversee library handling,

tracking, and storage.

* Use a keen attention to detail to maintain sample integrity and identification throughout

entire workflow.

* Strict adherence to local, regulatory, and quality management guidelines to ensure full


* Help guide technology transfer from MSSM R&D into production environment.

* Train new hires.


* Bachelor's degree in life science-related discipline.

* Experience in safe handling and disposal of potentially infectious clinical specimens in a biological safety level laboratory environment.

* 5+ years experience with standard molecular techniques:

PCR, qPCR, electrophoretic methods, DNA quantification, purification, and pooling methods, enzymatic manipulations, and primer design.

Preference will be given to those applicants with previous NGS library experience.

Experience with operating and programming liquid handling systems is preferred.

* 2+ years experience operating NGS systems. Experience with multiple platforms is a


* Experience with developing and/or performing high complexity lab-developed tests

(LDTs) is a plus.

* Experience in a CLIA/CAP-accredited facility is preferred.

About Our Organization

An extension of the Department of Genetics and Genomic Sciences and the Institute of Multiscale Biology in the Icahn School of Medicine at Mount Sinai, our state of the art next generation sequencing facility utilizes a variety of cutting edge, high throughput technologies. Our laboratory will generate huge amounts of sequencing data whose interpretation has the potential to change the face of how medicine is practiced. We will participate in large sequencing projects and analyze large numbers of individuals to gain a better understanding of the biology of cancer and inherited diseases. One of our initial focal points will be The Resilience Project where tens of thousands of DNA samples from healthy adults will be sequenced and analyzed for the presence of rare genetic variants that were expected to cause severe illness in these individuals during childhood.

We are looking for team members to join our dynamic, rapidly growing team who can help accelerate our growth and contribute to improving the accuracy of diagnoses, use of therapeutic agents, and achieve optimal patient outcomes. We seek all levels of laboratory and scientific personnel, informatics experts, accessioners, quality management specialists and professionals to fill a variety of important support functions. Be part of our excitement!

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