Head of Transgenic Facility

Organization
EMBL
Job Location
Monterotondo(near Rome)
Italy
Salary
Grade 6, 7 or 8, depending on experience and qualifications
Job Description

The European Molecular Biology Laboratory (EMBL) is one of the highest ranked scientific research organizations in the world. The Headquarters Laboratory is located in Heidelberg (Germany) and the outstations are in Grenoble (France), Hamburg (Germany), Hinxton (UK) and Monterotondo (Italy).

 

EMBL is looking for a Head of Transgenic Facility at the Mouse Biology Unit in Monterotondo, near Rome, Italy.

The postholder will report to the Head of the Outstation and he/she will be in charge of:

• Operation of the transgenic and mutagenesis facility, involving the generation of transgenic mice and mouse mutants for internal and external users. Genome engineering techniques used include microinjection of DNA (including large constructs), CRISPR/Cas9 and Talens approaches, and generation of chimera from targeted ES cells by injection into the morula and blastocyst stages.
• Organisation and supervision of breeding colonies of wild-type and mutant mice necessary for the work of the transgenic service under standard operating procedures.
• Supervision and implementation of protocols for the importation/quarantine of animals and establishment where necessary of a Specific Pathogen Free (SPF) barrier area involving embryo re-derivation.
• Management of a team of transgenic facility personnel in the area of animal husbandry, microinjection, surgery, ES cell culture, and related embryology and molecular biology techniques.
• Maintenance and updating of the online transgenic database for tracking work at the facility and the support of internal and external users.
• Conservation of mouse stocks by embryo and sperm cryopreservation.
• Application of IVF procedures for the rederivation of mouse lines from cryopreserved sperm
• Use of intracytoplasmic sperm injection (ICSI) for the establishment of large construct transgenic mouse lines and the recovery of senescent and/or infertile mouse lines.
• Establishment and coordination of training activities both internal and external to EMBL in the area of transgenesis.

Requirements

Applicants should hold a Bachelor degree in Biology, Animal Science, or a PhD in a related field. Experience of at least 5 years in transgenic technologies (DNA and ES cell injection, cryopreservation, embryo transfer) is essential and experience in ICSI would be an advantage. The successful candidate will have excellent interpersonal skills, demonstrated collaborative ability, and experience of supervision and management. Previous work in an international environment is preferred. Fluent spoken and written English is required. Italian is not required, but would be an asset.

How to Apply

Please apply online through www.embl.org/jobs

Here is a direct link to the position:

http://ig14.i-grasp.com//fe/tpl_embl01.asp?newms=jj&id=53863&aid=15470

Please refer to the job number: MR_00041

About Our Organization

EMBL is an inclusive, equal opportunity employer offering attractive conditions and benefits appropriate to an international research organisation. Please note that appointments on fixed term contracts can be renewed, depending on circumstances at the time of the review.

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