This position will support the DNA Sequencing Facility of the University of Wisconsin Biotechnology Center (UWBC), a core facility with 2.5 million dollars in revenue serving over 200 principal investigators representing more than 1000 individual scientists at the University of Wisconsin. The responsibilities of this position include: assisting the campus research and clinical community (including faculty, staff, graduate and undergraduate students) in project design for next generation sequencing, SNP analysis, and other specialized assays upon request; managing multiple customer projects and scheduling instrument runs and technical staff activities to complete those projects in a timely and cost effective manner, developing laboratory protocols to improve existing services or add new services; establishing and monitoring maintenance procedures for DNA sequencers, liquid handing robots, and related equipment; monitoring staff training and testing for compliance with CAP/CLIA regulations; attending and participating in on-campus meetings and seminars related to DNA isolation and DNA sequencing.
All staff are required to complete and stay current on HIPAA and Human Subjects training
5 - Monitor record keeping, equipment and lab maintenance, and staff training and testing on all Next Generation platforms and accessory equipment for compliance with CAP/CLIA regulations.
5 - Maintain complete records of all customer samples, derivatives of those samples, procedures performed on them, and resulting data.
20 - Oversee LTEs and Interns on a daily basis directing these individuals’ day-to-day activities and bench work. **Supervise four (at this time) permanent professional technicians in the DNA Sequencing Facility.**
5 - Assist the campus research community (including faculty,staff, graduate students, and undergraduate students) in experimental design and sample preparation for genotyping studies.
5 - Generate reports (molecular marker discovery/genotyping) and explain and disseminate information as requested.
5 - Prepare sample libraries and perform quality control for next generation sequencing (NGS).
5 - Establish and periodically update detailed standard operating procedures for our particular environment that maximize yield and quality.
5 - Research, evaluate, and optimize new methods/protocols for next generation sequencing services with regard to their cost, consistency, and throughput.
30 - Recommend to researchers appropriate designs for their NGS experiments for Illumina Illumina HiSeq 2500, Illumina MiSeq and Pacific Biosciences Sequel (replicates, appropriate controls, depth of coverage). **Discuss with PIs any updates with their sequencing projects, create quotes for services, and mediate any concerns that customers may have with their sequencing projects. Function as a consultant with in depth technical input related to the researcher's needs.**
5 - Validate quality of next generation sequencing runs against Illumina's published metrics (cluster counts and reads passing filter) to determine if any run errors justify rerunning client samples at no charge. Negotiate free replacement of consumables with Illumina in the event of substandard consumables or machine failures. Notify clients of these situations when they occur. Approve dissemination of good quality data to clients.
5 - Attend and participate in on-campus meetings and seminars related to DNA genotyping and next generation sequencing as well as any training seminars for equipment purchased in the future by the University of Wisconsin Biotechnology Center DNA Sequencing Facility.
5 - As the Lead Manager in the Next Gen Sequencing/Genomics Research Lab, promote the services that the core offers and match those services to the research needs of the UW faculty and outside researchers. Requires a high level of technical proficiency and an ability to internalize the research needs of an individual and/or group in order to provide the best services at a scientific level.
A successful background check is required prior to hiring.
This position is subject to a one year evaluation period.
A criminal background check will be conducted prior to hiring.
A period of evaluation will be required