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eCase Study Videos

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April 14, 2020
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SeraCare

Experience with SARS-CoV-2 (COVID-19) Assay Validation

Case Study

In this 20-minute eCase Study you will hear first-hand how Dr. Jeff SoRelle and team, of the University of Texas Southwestern Medical Center, developed and validated a COVID-19 RT PCR assay. Dr. SoRelle shares his decision to bring this development in house, how he chose reference materials, and how he managed to scale up and provide rapid turnaround.

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April 14, 2020
Sponsored by
SeraCare

Rapid Implementation of SARS-CoV-2 Testing

Case Study

In this 15-minute, on-demand video, Dr. Joel Lefferts describes how his team at the Dartmouth-Hitchcock Medical Center were able to rapidly increase, through automation, their SARS-CoV-2 assay on the Abbott m2000. Dr. Lefferts provides practical experiences and challenges faced when developing, validating, and going live with a SARS-CoV-2 assay to facilitate patient testing.

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Case Study

In this 15-minute on-demand presentation, learn how Accuplex SARS-CoV-2 Reference Materials are meeting the immediate need for validating the performance of coronavirus assays. The focus of this presentation is on the technology, the importance of early detection, and helping every lab accelerate go-live-launch.

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April 02, 2020
Sponsored by
Thermo Fisher Scientific

Critical Raw Material for mRNA-based Vaccine Development

Case Study

Accelerated development of mRNA-based vaccine has necessitated the transformation of standard, research grade in vitro transcription reagents into raw material specifically designed and manufactured under advanced quality standards. The TheraPure grade reagents for mRNA-based vaccine represents Thermo Fisher Scientific’s highest level of purity for mRNA synthesis reagents, with products manufactured using high-definition analytics and tightly controlled purity standards. The TheraPure grade portfolio offers a unique integrated solution for mRNA synthesis, including animal origin-free and ampicillin-free enzymes.

Here, we will discuss the quality requirements for critical raw material for commercial mRNA production and ongoing projects to optimize the entire mRNA portfolio to meet fit-for-purpose standards.

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Case Study

Conventional animal testing for quality control of vaccines is lengthy and may require large numbers of animals. To address these limitations, the use of specific biomarkers to evaluate batch-to-batch consistency and the safety of vaccines has been proposed. Specific panels could also be used as novel markers to evaluate adjuvant safety profiles and for safety assessment of inoculation routes.

The QuantiGene multiplex gene expression assays are based on branched DNA (bDNA) technology and allow for direct-from-sample transcriptional profiling.  These assays have been used to develop more reliable and robust parameters for testing than conventional animal tests. In addition, with the advent of nucleic acid vaccines such as mRNA vaccines, the QuantiGene technology has been used to evaluate the safety of delivery systems and to monitor the biodistribution of these nucleic acids after administration via different routes. These are important aspects to be determined to demonstrate that the safety profiles of these RNA vaccines are comparable to traditional vaccines.

Here we discuss some of these recent advances in evaluation of vaccine quality control and safety utilizing the QuantiGene multiplex gene expression assays.

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April 02, 2020
Sponsored by
Thermo Fisher Scientific

From Synthetic Gene to Vaccine: Confidence with Gene Synthesis Services

Case Study

Synthetic biology accelerates vaccine development by providing scientists an efficient mechanism to quickly design and test diverse antigen sequences to create favorable vaccine characteristics such as enhancing antigen immunogenicity and improving vaccine stability. Once candidate sequences are determined, GeneArt Gene Synthesis services provide a range of de novo gene synthesis options to fast-track discovery, key to rapid vaccine development.

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April 02, 2020
Sponsored by
Thermo Fisher Scientific

Harnessing the Power of Protein Expression Systems in Vaccine Development

Case Study

In just a few months COVID-19 has risen from a regional crisis to a global threat, and drug makers are now scrambling to develop vaccines aimed at thwarting a pandemic. To meet this urgent and life-threatening situation, vaccine developers are actively seeking the most efficient and robust production platforms to accelerate development timelines. Throughput, productivity, and scalability of the expression platform as well as the availability of well-documented production cell lines are key selection criteria when initiating a vaccine development campaign under such urgent circumstances. To this end, the Gibco Expi Expression Systems are complete platforms that can accelerate vaccine development by enabling rapid, high-yield, and scalable production of proteins, viral antigens and viral-like particles from mammalian (ExpiCHO and Expi293) and insect (ExpiSf) cells. By providing flexible and highly productive expression systems for three different cell hosts that employ optimized and chemically defined components, the Expi systems support every stage of vaccine development. This unified, end-to-end approach reduces key product quality risks during development as one can use the same cell line from research to large-scale production while streamlining process development by utilizing integrated reagents for unmatched performance.

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March 20, 2020
Sponsored by
Thermo Fisher Scientific

Enhanced, Rapid Target Identification by NGS for Vaccine Development

Case Study

Next-generation sequencing (NGS) is the primary technology used to identify genomic targets for vaccine development. Enhanced options for NGS on Illumina systems now make it possible to identify multiple potentially pathogenic strains of a disease and understand the rate of evolution quickly, with improved precision. Two complementary sequencing methods are used to identify targets for vaccine development. Whole-genome sequencing using the Invitrogen Collibri DNA Library Prep Kits provide the entire genomic assembly, offering an improvement over older transposomic methods. Collibri 3ʹ mRNA Library Prep kits provide gene expression insights into host-pathogen interactions that can be used to refine genomic targets for vaccine development.

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Case Study
In this on-demand video presentation, Dr. Keith Gligorich, laboratory operations director at Navican Precision Cancer Care, discusses his experiences implementing a next-generation sequencing quality management system at Navican. 
 
Download the video to hear Dr. Gligorich explain the details of Navican's NGS quality management system and how it addresses the complexities of the precision medicine workflow.  
 
 
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New analyses indicate female researchers are publishing less during the coronavirus pandemic than male researchers, according to Nature News.

A study suggests people with the ApoE e4 genotype may be more likely to have severe COVID-19 than those with other genotypes, the Guardian says.

Direct-to-consumer genetic testing companies are searching for a genetic reason for why some people, but not others, become gravely ill with COVID-19, the Detroit Free Press reports.

In PNAS this week: forward genetics-base analysis of retinal development, interactions of T cell receptors with neoantigens in colorectal cancer, and more.