Waters introduced this week version 2.2.5 of its ProteinLynx Global Server software. The new software allows researchers to identify and quantify proteins more efficiently, the company said.
PLGS 2.2.5 has improved algorithms for performing quantitative proteomics without the use of isotope labeling technologies, said Waters. In addition, the software has a new quantification module that allows quantitative proteomics data to be generated using any commercially available labeling technology, such as SILAC, AQUA, ICAT, or iTRAQ, or any user-defined labeling technology.
For protein identification, PLGS 2.2.5 incorporates a new algorithm that enables identification of proteins from MSE, or elevated collision energy data, such as the data acquired from a Q-TOF mass spectrometer. A proprietary "parallel" peptide fragmentation protocol is used in LC/MS experiments. The protocol results in higher sequence coverage and confidence in protein identification than traditional "data-dependent acquisition" MS/MS methods, Waters said.
Other enhancements for PLGS 2.2.5 include new data visualization tools that visually depict what happens to protein concentration over a large number of samples or conditions.
PLGS 2.2.5 is compliant with mzXML and mzData data formats.
Syngene announced this week the availability of its new automated 2D gel imager, Dyversity.
Dyversity consists of a light-tight darkroom containing a high-resolution, 16-bit CCD camera with 90 microns of resolution. The darkroom has a fast capture time, and features a large door opening to accommodate many gel sizes, the company said.
Dyversity can be fitted with a range of precision-made filters, as well as UV and white lighting modules to allow imaging of protein stains such as coomassie blue, deep purple, Pro-Q Diamond, silver stain, and SYPRO Ruby. In addition, there is a Cy dye lighting platform available.
Results produced by Dyversity can be transferred into Syngene's new Dymension software for image analysis.
The BioPAX (Biological Pathways Exchange) workgroup has released BioPAX Level 2 v1.0 at http://www.biopax.org. The release supports representation of metabolic pathways, molecular interaction databases, and "some aspects" of signal transduction, such as protein post-translational modifications, according to an announcement from the workgroup. Subsequent levels will support signaling pathways, gene regulatory, and genetic interaction networks
Movers & Shakers
Mark Bloomfield joined Applied Biosystems as vice president of sales for molecular biology in Europe in November 2005, ABI said this week.
Bloomfield most recently was director of European commercial operations for the Scientific Instruments division of Thermo Electron. He has also served as aftermarket sales and sales development manager with Agilent Technologies Europe and European business development manager for mass spectrometry and gas phase chromatography with Hewlett-Packard.