At the Human Proteome Organization’s annual conference in Amsterdam this week, Sigma-Aldrich announced the addition of 2,000 antibodies to its Prestige Antibodies line of highly characterized monoclonal antibodies.
The antibodies are immediately available in Europe and expected to be available in the US and other markets on Sept. 1.
The Prestige line now has more than 3,800 antibodies directed against 3,600 genes. The antibodies were developed by the Human Proteome Resource and are made available through a partnership between Sigma-Aldrich and Atlas Antibodies, the commercial arm of the HPR. The antibodies are characterized by the Human Protein Atlas, part of HPR.
Also at HUPO, Thermo Fisher Scientific launched the Automated Phosphopeptide Workflow and upgraded versions of its Sieve and ProSight software packages for proteomics.
A complete protocol for the automated analysis of phosphopeptides, Automated Phosphopeptide combines Thermo Scientific technologies with the Pierce Phosphopeptide Enrichment kit, the Kingfisher Flex magnetic beat purification system and the LTQ Orbitrap XL ETD hybrid MS “to enable fully automated analysis of phosphopeptides,” the company said in a statement.
Sieve 1.2 label-free differential analysis software allows researchers to analyze multiple time points or points within a single trend. The software automates the label-free expression of differential expression of proteins and peptides by comparing LC/MS datasets of healthy or control sets with treated or disease samples to determine whether differences exist. It does so without the need for isotopic labels, the company said.
ProSight PC 2.0 now supports the identification and characterization of proteins from all high mass accuracy tandem mass spec experiments. It enables high-throughput processing of all accurate mass MS2 data and includes the characterization of proteins with known post-translational modifications. The software also includes a new graphical interface for improved ease-of-use and offers automatic LC-MS file processing, the company said.
Separately, Thermo Fisher announced the launch of the Sorcerer Enterprise software that it co-developed with Sage-N-Research.
The software, available from Sage-N-Research, is a scalable software suite for fully automated, high-volume proteomic analyses on Linux systems. It can be used as a standalone data-analysis system or be interfaced with Thermo’s recently launched Proteome Discoverer software.
Sorcerer is the latest product of a five-year collaboration between Thermo Fisher and Sage-N-Research begun in 2003.
The newest version of the Human Protein Atlas was also released at HUPO this week.
The release was the fourth version of the HPA and includes 6,121 antibodies, 5,076 protein-encoded genes, and more than 5.7 million images, doubling the output from a year ago when the third version of the atlas was launched. The new version now represents a quarter of all the estimated protein-encoded genes in humans, said Matthias Uhlen, who spearheaded the HPA.
Changes to the atlas include a “gene-centric focus” and the display of antigen sequences for each protein, Uhlen said. The atlas also now includes protein classes, both functional and project-related.
An antibody portal for sharing antibody data, Antibodypedia, was also released in conjunction with the updated HPA.
Started in 2005, the atlas is a database of images of protein expression in tissues in normal state and some cancer states. Since the original launch of 718 antibodies and more than 415,000 images, the size of the atlas has doubled each year.
Promega plans to introduce protein arrays at the end of the year, marking its entry into the market, company officials told ProteoMonitor this week.
The arrays, based on the company’s HaloTag technology, will be available on demand. The arrays are targeted for validation work, not screening, and no content will be provided.
The arrays will have about 50 micro-wells per slide though customers will be able to spot higher density as desired. Pricing has not been determined yet, but “we’ll do it so that it’s not cost-prohibitive for the academic researcher,” said Hrissi Samartzidou, global director for gene and protein sciences at Promega.
Pressure BioSciences launched ProteoSolve-SB, a kit for the simultaneous extraction, isolation, and fractionation of proteins, nucleic acids, and lipids from animal and plant samples.
The kit uses Pressure BioSciences pressure cycling technology and contains proprietary reagents and Pulse consumable processing containers.
Bioinquire this week released version 1.1 of its ProteoIQ software for the analysis of proteomic data.
The new version allows users to customize how MS/MS data are displayed, including annotation of ion series labels, charge states, and enhanced screening functionality for identifying neutral losses. Annotated MS/MS spectra for identified peptides can be exported and proteomic results can be saved in a format that conforms with the Paris Guidelines for the Reporting of Protein Identification Data, the company said in a statement.