A team of Roche researchers has developed a five-plex SILAC method for quantifying the dynamics of tyrosine phosphorylation.

The method, which was detailed in a paper published last week in Molecular & Cellular Proteomics, expands SILAC labeling from the traditional three-plex to five-plex by using a mixture of light and heavy isotopically labeled lysines, arginines, and tyrosines to divide a sample into five distinct populations.

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