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Recent Research Papers of Note: Dec 10, 2010

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Proteomics research papers of note, November 2010

Journal: Molecular & Cellular Proteomics, Nov. 3 [Epub ahead of print]

Title: Accurate quantification of more than 4,000 mouse tissue proteins reveals minimal proteome changes during aging.

Authors: Walther DM; Mann M.

The authors performed an in-depth proteomic analysis of three brain regions as well as hearts and kidneys of mice aged five or 26 months using SILAC and high-resolution mass spectrometry. Less than 1 percent of all proteins showed mean abundance changes of more than two-fold between young and old mice, suggesting that aging-related effects on the tissue proteome composition are minor.


Journal: Journal of Proteome Research, Nov. 5

Title: Time-dependent proteomic iTRAQ analysis of nasal lavage of hairdressers challenged by persulfate.

Authors: Kåredal MH; Mortstedt H; Jeppsson MC; Kronholm Diab K; Nielsen J; Jonsson BA; Lindh CH.

The researchers used iTRAQ labeling and LC-MS/MS to compare protein expression in the nasal lavage fluid proteome upon exposure to potassium persulfate in hairdressers with and without bleaching powder-associated rhinitis. Differences in protein expression were observed, particularly in proteins tied to lipid transportation and antimicrobial activities.


Journal: Molecular & Cellular Proteomics, Nov. 7 [Epub ahead of print]

Title: Confident phosphorylation site localization using the Mascot Delta Score

Authors: Savitski MM; Lemeer S; Boesche M; Lang M; Mathieson T; Bantscheff M; Kuster B.

The authors evaluated the Mascot Delta Score as a simple method for identifying site localizations for phosphorylation and examined the ability of different fragmentation techniques to localize phosphorylation sites. They found that Mascot typically achieved similar sensitivity and specificity for localization as the published Ascore.


Journal: Journal of Proteomics, Nov. 12 [Epub ahead of print]

Title: Untangling the intracellular signaling network in cancer — A strategy for data integration in acute myeloid leukemia

Authors: Jørgensen KM; Hjelle SM; Oye OK; Puntervoll P; Reikvam H; Skavland J; Anderssen E; Bruserud O; Gjertsen BT.

The researchers propose a partial least squares regression-based data integration strategy to combine proteomic data, gene expression data, and classical clinical data into one analysis with the aim of tying complicated, multidimensional datasets to interpretations of the underlying biology.


Journal: Journal of Proteome Research, Nov. 18 [Epub ahead of print]

Title: Quantitative analysis of human cerebrospinal fluid proteins using a combination of cysteine tagging and amine-reactive isobaric labeling

Authors: Giron P; Dayon L; Turck N; Hoogland C; Sanchez JC.

The authors present a strategy for cysteine peptide enrichment using successive cysteine and amine-isobaric tagging. They applied to technique to human cerebrospinal fluid from post-mortem and ante-mortem samples, in which they were able to quantitate cysteine-containing peptides with coefficients of variation and mean absolute errors of less than 10 percent.


Journal: Journal of Proteomics, Nov. 27 [Epub ahead of print]

Title: Integration of proteomic analyses to monitor the activity status of phosphorylation signaling

Authors: Nagano K; Shinkawa T; Yabuki N; Mutoh H; Inomata N; Watanabe Y; Ashihara M; Nagahashi S; Ishii N; Aoki Y; Haramura M.

The authors combined metal oxide affinity chromatography and anti-phosphotyrosine antibody phosphoproteomics to enhance coverage of phosphorylated peptides in a mass spec analysis of Erk signaling.


Journal: Proceedings of the National Academy of Sciences, Nov. 29 [Epub ahead of print]

Title:
{alpha}-Dystrobrevin-1 recruits {alpha}-catulin to the {alpha}1D-adrenergic receptor/dystrophin-associated protein complex signalosome

Authors: Lyssand JS; Whiting JL; Lee KS; Kastl R; Wacker JL; Bruchas MR; Miyatake M; Langeberg LK; Chavkin C; Scott JD; Gardner RG; Adams ME; Hague C.

The authors performed a proteomic analysis of the dystrophin-associated protein complex in order to test their hypothesis that the DAPC imparts functionality to the α(1D)-adrenergic receptor signalosome by recruiting previously unidentified molecules. The analysis detecting a common interacting protein, α-catulin, implicating it as a regulator of α(1D)-adrenergic receptor signaling.


Journal: Chinese Journal of Hepatology

Title: Subcellular proteome analysis of immune or alcohol induced rat liver fibrosis, Nov. 2010

Authors: Jia XF; Peng X; Feng YL; Yang H; Yuan ZH; Zhang LJ.

The researchers used LC-MS/MS to identify proteins differentially expressed in rats due to immune or alcohol related liver fibrosis. The study identified 30 differentially expressed proteins including annexin A2, keratin 8, and keratin 19, which, the authors noted in the abstract, could have potential as fibrosis biomarkers.

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Guidelines for Ancient DNA Work

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And Cleared

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Suit Over Allegations

The Boston Globe reports that David Sabatini, who was placed on leave from MIT after allegations of sexual harassment, is suing his accuser, the Whitehead Institute, and the institute's director.

Nature Papers on Esophageal Cancer, Origin of Modern Horses, Exome Sequencing of UK Biobank Participants

In Nature this week: genetic and environmental influences of esophageal cancer, domestic horse origin traced to Western Eurasian steppes, and more.