The method described exposes electrospray droplets or solid sample matrices to an ion beam “thereby increasing the unbalanced charge of the analyte,” according to the abstract. In another embodiment, a method is provided in which ionization of the sample is achieved by directing an ion beam at a liquid or solid sample matrix containing the analyte, ionizing, and adding an unbalanced charge to the analyte.
Recent Patents of Interest in Proteomics: Jan 8, 2009
US Patent 7,473,893. ICP/ESI mass spectrometry systems and methods of use thereof. Inventors: Viorica Lopez-Avila; Ganqiang Li. Assignee: Agilent Technologies
Invention pertains to mass spectrometry systems and methods for performing molecular mass spectrometry and elemental mass spectrometry using a single mass-spec system.
US Patent 7,473,535. Chemical reagents and methods for detection and quantification of proteins in complex mixtures. Inventors: Rudolf Aebersold; Patricia Bottari; Michael Gelb; Frantisek Turecek. Assignee: The Institute for Systems Biology; University of Washington
Provided are reagents comprising an affinity tag, a detectable moiety, a linker, an isotope tag, and a reactive group. Methods for using the reagent of the invention are also provided. The methods can be used to label a polypeptide in a sample by contacting a sample with a reagent of the invention under conditions which would allow the reactive group to bind to at least of the polypeptides in the sample. Methods for isolating, identifying, and quantifying a polypeptide in a sample are also provided, as are methods for diagnosing diseases using a reagent of the invention.
US Patent 7,470,778. Use of ionic liquids for protein extraction. Inventors: Jeorg von Hagen; Uwe Michelsen. Assignee: Merck Patent
Invention pertains to a method for extracting proteins, protein fragments, and peptides from biological samples. The extractants are ionic liquids of the general formula K+A-. A kit and uses of it are also described.
US Patent 7,470,540. Method and device for the integrated synthesis and analysis of analytes on a support. Inventors: Cord Stahler; Ramon Guimil; Matthias Scheffler; Peer Stahler; Anke Heidbrede. Assignee: Febit
A method for preparing receptor-coated particles is described. It consists of six steps beginning with a support body and includes conducting liquid containing particles into or onto the support body and immobilizing the particles on at least one surface area of the support body.
US Patent 7,468,160. Devices and methods for performing array-based assays. Inventors: Allen Thompson; George Tsai; Russell Alan Parker. Assignee: Agilent Technologies
The subject array assay includes a base, cover, and clamping member for holding the cover to base. Embodiments of the subject methods include “contacting a sample with a backing element and placing the backing element supported sample in contact with an array assembly to form a structure that includes the backing element and array assembly,” according to the abstract.
US Patent 7,463,357. Wide dynamic range chemical array reader. Inventors: John Kralik; Tariq Faiz. Assignee: Agilent Technologies
Described is a system for detecting a predetermined wavelength of light emitted from an area on a chemical array. Also provided are methods for detecting light using the subject system, programming for performing the subject methods, and an array reader containing the subject system.
US Patent 7,462,823. Method for increasing ionization efficiency in mass spectroscopy. Inventor: Luke Schneider. Assignee: Target Discovery