US Patent No. 7,041,459. Analyzing phosphorylated proteins. Inventors: Sharat Singh, Robert Zivin. Assignee: Monogram Biosciences.
This invention discloses methods, compositions and kits for determining one or more target polypeptides in a sample where target polypeptides have undergone phosphorylation. The method uses an immobilized metal affinity chromatography, or IMAC support specific for phosphate groups on one or more target polypeptides.