The invention provides for polynucleotides and vectors comprising at least two tag sequences, and provides for polynucleotides and vectors comprising a streptavidin binding peptide sequence, and a calmodulin binding peptide sequence. It provides methods for using the polynucleotides and vectors of the invention for detecting and isolating protein complexes or identifying a binding partner for a protein of interest.
Recent Patents of Interest in Proteomics: Jul 19, 2007
US Patent 7,244,411. Method of selective peptide isolation for the identification and quantitative analysis of proteins in complex mixtures. Inventors: N La Nez; Jorge Ferna de Cossio Dorta-Duque; Vladimir Armando Besada Perez; Jeovanis Gil Valdes; Luis Javier Gonza Lopez; Gabriel Ramoo Palomares; Rolando Pajo Feyt; Fe Gil
The method comprises the selective isolation of peptides that contain neither arginine nor histidine and determination of the relative concentrations of at least one protein in different samples from the ratio between the areas of the “estimated theoretical spectra for the NHNR peptides labeled with different isotope in each sample,” according to the abstract.
US Patent 7,244,396. Method for preparation of microarrays for screening of crystal growth conditions. Inventors: Arnon Chait; Lawrence DeLucas; Brad Stoops; Alexander Belgovskiy. Assignee: The University of Alabama, Birmingham Research Foundation.
The method comprises preparing a plurality of recipe solutions in milliliter volumes, aspirating and dispensing protein and recipe solutions from several wells, covering the combined protein and recipe solutions with oil, and maintaining the solution wells until crystallization or precipitation occurs.
US Patent 7,243,112. Multidimensional biodata integration and relationship inference. Inventors: Kunbin Qu; Nan Lin; Yanmei Lu; Donald Payan. Assignee: Rigel Pharmaceuticals
Invention pertains to a platform for analysis of biological data. The platform emphasizes pathway mapping and relationship inference based on data acquired from multiple diverse sources. “It uses a bioinformatics system that integrates data from the diverse sources, connecting related genes and proteins and inferring biological functions in the context of global cellular processes,” according to the abstract.
US Patent 7,236,891. Methods of determining polypeptide structure and function. Inventors: Peter Davies; John Elce; Christopher Hosfield; Zongchao Jia; Tudor Moldoveanu. Assignee: Queen’s University at Kingston, Ontario
Invention pertains to methods for determining the structure and/or function of one or more domains of a cation-dependent polypeptide in the presence of one or more cation. The invention is aimed particularly toward calcium-dependent cations and peptides that are calcium-dependent enzymes. It also provides methods for identifying ligands with the ability to bind to one or more ligand-binding domains of a cation-dependent polypeptide, and ligands identified by these methods.
US Patent 7,235,781. Laser system for the ionization of a sample by matrix-assisted laser desorption in mass spectrometric analysis. Inventors: Andreas Haase; Markus Kayser; Jens Hohndorf; Armin Holle. Assignee: Bruker Daltonics.
The invention consists of a laser system that generates a spatially modulated intensity distribution with intensity spots on the sample. This improves the quality and robustness of the mass spectrometric analysis and the ionization efficient, and for TOF MS with axial injection, the mass resolution and the signal-to-noise ratio.
US Patent 7,235,362. Compositions and methods for protein isolation. Inventors: Jeffrey Braman; Carsten-Peter Carstens; Natalia Novoradovskaya; Rajesh Bagga; Lee Basehore. Assignee: Stratagene.