Qiagen has released two new assay kits for detecting tyrosine kinase and serine/threonine kinase activity without radioactivity. The LiquiChip Broad-Range Kinase kits consist of kinase substrates immobilized on LiquiChip beads. The beads are incubated with a sample containing inactive kinases and a non-radioactive cocktail that serves as a phosophate donor. Once the reactions are complete, primary antibodies recognizing phosphorylated serine, threonine, or tyrosine detect the phosphorylation sites.
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