Qiagen has released two new assay kits for detecting tyrosine kinase and serine/threonine kinase activity without radioactivity. The LiquiChip Broad-Range Kinase kits consist of kinase substrates immobilized on LiquiChip beads. The beads are incubated with a sample containing inactive kinases and a non-radioactive cocktail that serves as a phosophate donor. Once the reactions are complete, primary antibodies recognizing phosphorylated serine, threonine, or tyrosine detect the phosphorylation sites. A biotinylated secondary antibody detects the primary antibody, and the secondary antibody is then detected by a fluorescent marker.
NanoHorizons and Kratos Analytical have jointly released a matrix-less MALDI target for small molecule analysis called QuickMass. The company will distribute the targets through Shimadzu Biotech. According to the companies, QuickMass increases selectivity and reduces cross-contamination for small molecule detection in MALDI-MS. The increased selectivity and lower cross-contamination results from QuickMass’ non-porous, thin film surface, which adsorbs and transfers the laser energy to the sample molecules, according to the companies.
Amersham Biosciences has released a suite of bioprocess membrane separations systems called UniFlux. The suite is available in two forms: a fully automated system powered by software and designed to automatically control and respond to process events; and a Windows-based PC data-logging version for those who want to maintain manual control. The system provides greater efficiency and lower costs than systems in which membrane separations and chromatography are not under unified control, the company said.