US Patent No. 915,891. Method and apparatus for unloading gradients. Inventor: Norman Anderson. Assignee: Large Scale Proteomics.
A float is formed with a concave upper surface defining a well. The float is adapted for insertion into a vessel such as a centrifuge tube that contains a density gradient therein. The float may be pushed downward in the tube to allow a portion of the density gradient to spill over an upper edge of the float. A pipetter may then be used to remove the separated portion of the density gradient now located in the well. By repeatedly pushing the float successively lower into the tube, various portions of the density gradient may be easily separated and removed.
US Patent No. 6,761,810. Automated apparatus including a robotic arm for loading samples into wells for first dimension electrophoresis separation. Inventors: Andrew McGrath, Leigh Anderson, Jack Goodman. Assignee: Large Scale Proteomics.
This invention is an automated apparatus for first dimensional isoelectric focusing of proteins and other macromolecules. The automated apparatus includes a supply magazine, an electrophoresis tank and an automated transferring device that robotically transfers biological samples from sample vials held in the supply magazine to tube gels supported in a rack within the electrophoresis tank. The transferring device is configured to move in three dimensions with respect to the supply magazine.
US Patent No. 412,191. Mass spectrometer autosampler. Inventors: Ming Gu, Wenjeng Li, John Allen. Assignee: Bristol-Myers Squibb Company.
This invention is an autosampler device that is useful for the automated introduction of small sample volumes into a high pressure liquid chromatography system. In addition, the device can also be used for analyzing low-abundant protein samples.
US Patent No. 6,777,239. Epitope-captured antibody display. Inventors: William Dower, Steven Cwirla. Assignee: XenoPort
This invention includes reagents and methods for detecting target proteins in a sample. The reagents include a replicable genetic package and a protein displayed on an exterior surface of the package that was expressed from a nucleic acid within the package. In addition, one or more antibodies are complexed to the expressed protein, which have an open binding site for a target protein. The reagents are used individually, or as part of a library or array to bind target proteins within samples. By determining the sequence of the nucleic acid fragment within the complex, one can identify the target protein since the fragment encodes for an epitope that is shared by the expressed and target proteins.
US Patent No. 574,692. Arrays of protein-capture agents and methods of use thereof. Inventors: Peter Wagner, Steffen Nock, Dana Ault-Riche, Christian Itin. Assignee: Zyomyx.
This patent covers arrays of protein-capture agents that are useful for the simultaneous detection of a number of proteins, in parallel, that are expression products of a cell or population of cells. The invention consists of at least one organic thinfilm covering the surface of the substrate, and a number of patches arranged in discrete, known regions. Each patch has protein-capture agents immobilized on the organic thinfilm.
US Patent No. 053,098. Integrated high throughput system for the mass spectrometry of biomolecules. Inventors: Kemmons Tubbs, Karl Gruber, Randall Nelson.
This patent covers an affinity microcolumn comprised of a high surface area material which has high flow properties and a low dead volume. The high surface area material has affinity reagents bound to it that are either activated or activatable. The column aims to provide a system capable of retrieving and concentrating specific biomolecules from biological media for subsequent analyses, such as quantifying of targeted proteins, recognizing of variants of targeted biomolecules, or elucidating the nature of ligands interacting with targeted biomolecules.
US Patent No. 6,787,330. Method and kit for identifying or characterizing polypeptides. Inventors: Willy Bienvenut, Denis Hochstrasser, Jean-Charles Sanchez. Assignee: University of Geneva.
This patent covers polypeptides which have been separated by gel electrophoresis. The polypeptides can be identified by a procedure that has two main stages: In the first stage, the gel is digested with a cleaving agent such as an enzyme. This produces mainly large fragments, which in the second stage are electroblotted through a hydrophilic membrane onto a hydrophobic membrane. The resulting fragments, usually peptides, are identified by MALDI-TOF mass spectrometry, or by interaction with an antibody.