US Patent No. 6,963,807. Automated identification of peptides. Inventors: Robert Townsend, Andrew Robinson. Assignee: Oxford Glycosciences [now Oxford Genome Sciences].
This invention relates to computer-mediated methods for automated interpretation on data obtained by mass spectrometry. The method identifies a corresponding peptide sequence if it is present in a peptide database, without the need for a skilled observer to choose from amongst a list of possible matches. The method also permits mapping of mass spectral data to sequences in peptide or nucleotide databases for unambiguous identification of exons; determining a correct reading frame; identifying artifacts and errors in sequences; identifying mutations and polymorphisms; identifying post-translational modifications; and identifying exon-intron boundaries.
US Patent No. 6,969,757. Differential labeling for quantitative analysis of complex protein mixtures. Inventors: Paul Haynes, Jing Wei, John Yates, Nancy Andon. Assignee: Syngenta Participations.
This inventions relates to a method for simultaneously identifying and determining the levels of expression of cysteine-containing proteins in normal and perturbed cells.
US Patent No. 6,969,614. Methods for the isolation and analysis of cellular protein content. Inventors: Lance Liotta, Nicole Simone, Michael Emmert-Buck, Emmanuel Petricoin. Assignee: The United States of America as represented by the Department of Health and Human Services.
This invention describes devices and methods for performing protein analysis on laser capture microdissected cells. In addition to permitting direct comparison of qualitative and quantitative protein content of tumor cells and normal cells from the same tissue sample, the methods also allow for investigation of protein characteristics of tumor cells, such as binding ability, amino acid sequence, and differential expression of proteins in particular cell populations in response to drug treatment. The methods also provide, through the use of protein fingerprinting, a rapid and reliable way to identify the source tissue of a tumor metastasis.
US Patent No. 6,969,452. Two-dimensional protein separations using chromatofocusing and multiplexed capillary gel electrophoresis. Inventors: Yan He, Ho-Ming Pang, Siquan Luo, Futian Han. Assignee: Combisep.
This invention discloses a two-dimensional protein separation method. The method is based on separating a protein sample by chromatofocusing into a number of aliquots, then loading each aliquot into a separate capillary tube, and separating each aliquot by multiplexed capillary electrophoresis to produce a two-dimensional array of separated proteins.