US Patent No. 6,653,151. Dry deposition of materials for microarrays using matrix displacement. Inventors: Leigh Anderson, Norman Anderson, James Braatz. Assignee: Large Scale Proteomics.
Covers a method for manufacturing microarrays, including protein arrays, by sectioning bundles of tubules or rods containing the molecules to be arrayed immobilized in a predefined pattern in a matrix to produce large numbers of sample chips. The chips are then processed by deposition to microarrays. Using the covered method, the immobilizing matrix can then be partitioned away from the molecules and these molecules can be placed onto various surfaces for analysis by binding assays, for hybridization reactions, for diagnostic methods, and for a variety of cell interaction-determining methodologies.
US Patent No. 6,653,135. Dynamic protein signature assay. Inventor: Brian Bradley. Assignee: University of Maryland.
Covers an assay determining the protein profile for a single organism or population of organisms exposed to an environmental stress. The organism or organisms are assayed first to determine a set of proteins expressed and a second set of proteins suppressed in reponse to the stressor. These protein profiles can be statistically analyzed to determine which proteins expressed or suppressed would be useful as a diagnostic. Protein expression signatures can be determined describing the quantitative differences in protein profiles for an organism in response to a particular stressor in comparison to other stressors. A biological sample can then be tested for exposure to the stressor by using the protein expression signature for that particular stressor as a template.
US Patent No. 6,653,076. Stable isotopic metabolic labeling for analysis of biopolymers. Inventors: Robert Franza Jr., Yvan Rochon. Assignee: Regents of the University of Washington.
Covers methods for determining the rate of biopolymer synthesis and degradation in cells, tissues, or cell-free systems using non-radioactive stable isotope metabolic labeling. The polymer of interest is allowed to assemble in the presence of the label, and then the isotopic peaks can be measured by mass spectrometry. The relative abundance of the different mass peaks from samples containing probe is compared to samples not containing the probe, allowing the relative proportion of polymer synthesized to be determined.
US Patent No. 6,652,724. Automated apparatus for separating a biological sample from a two dimensional electrophoresis gel. Inventors: Samuel Michael, Jack Goodman, Leigh Anderson. Assignee: Large Scale Proteomics.
Covers an automated system for picking and identifying protein spots in a 2D gel. The system includes a computer controlled robotic arm and a sample plate handling assembly for supplying a sample plate to a loading station. A removable tray supports the slab gel during the cutting process and a sample plate handling assembly delivers a multiwell plate to the cutting assembly for receiving the gel spots. The removable tray also cooperates with the scanner for identification of the spots.
US Patent No. 6,649,351. Methods for detecting a plurality of analytes by mass spectrometry. Inventors: Tracy Matray, Vincent Hernandez, Ahmed Chenna, Herbert Hooper, Sharat Singh. Assignee: Aclara Biosciences.
Covers a method for detecting one or more target analytes by the following process: the analytes are put in contact with a set of first binding reagents containing a cleavage-inducing moiety and a target binding moiety; and a second set of binding reagents containing a tagged probe with a mass modifier region attached to a target binding moiety that is susceptible to cleavage. The cleavage-inducing moiety is activated to release the tag reporter, and the mass of that reporter is detected, indicating the presence of a unique known target analyte.
US Patent No. 6,657,191. Means and method for multiplexing sprays in an electrospray ionization source. Inventor: Melvin Park. Assignee: Bruker Daltonics.
Covers a means and method for multiplexing samples from multiple sprayer devices for transfer to a mass analyzer. Sample sprays from a plurality of sprayers are desolvated and sample ions are selected from one sprayer for entry into the analyzer via a multi-part capillary, the first section of which is moved from one sprayer to the next by use of a motor. This first section may be a flexible tube-like structure mounted on the aperture of a cone-shaped end of a motor that rotates so that it can be aligned with different sprayers at different times.
US Patent No. 6,653,622. Ion fragmentation by electron capture in high-frequency ion traps. Inventor: Jochen Franzen. Assignee: Bruker Daltonik.
Covers procedures and devices for fragmenting large molecules in high-frequency quadrupole ion trap mass spectrometers. Electrons are injected as a beam through an aperture in the ion trap electrode carrying the RF voltage, whereby the electron source is kept at the highest positive potential achieved at the center of the ion trap during the RF cycle.
The electrons can reach the ions only during a short period of a few nanoseconds of very low energy, at which point the ions are fragmented by electron capture.