US Patent No. 6,804,410. System for optimizing alignment of laser beam with selected points on sample in MALDI mass spectrometer. Inventors: John Lennon, Athony Makusky, Samuel Michael. Assignee: Large Scale Proteomics.
A mass spectrometry apparatus uses image processing of output signals of a camera in a mass spectrometer to provide feedback for directing a laser. The invention provides for the determination of where samples have been deposited on a plate, and for the selection of different points for each sample, based on its structure, at which to aim a laser, during the cycle period of the mass spectrometer. Such feedback information increases the likelihood that the laser impinges samples and provides useful data.
US Patent No. 6,803,568. Multi-channel microfluidic chip for electrospray ionization. Inventors: Luc Bousse, John Stults. Assignee: Predicant Biosciences.
In this invention, a microfluidic chip formed with multiple fluid channels terminating at a common electrospray ionization tip is used for mass spectrometric analysis. The fluid channels may be formed within a substrate plate that are in fluid communication with corresponding reservoirs. The electrospray tip can be formed along a defined portion of the substrate plate. The tip includes an open region at which the fluid channels converge.
The invention can provide methods for conducting mass spectrometric analysis of multiple samples flowing through individual fluid channels in a single microfluidic chip that is formed with a convergent electrospray tip.
US Patent No. 6,803,566. Sampling probe for microarray read out using electrospray mass spectrometry. Inventor: Gary Van Berkel. Assignee: UT-Battelle.
This invention includes an automated electrospray based sampling system where the method for analysis obtains samples from surface array spots having analytes. The system includes at least one probe that includes an inlet for flowing at least one eluting solvent. The system also includes an outlet for directing the analyte away from the spots. An automatic positioning system is provided for translating the probe relative to the spots to permit sampling of any spot. An electrospray ion source receives analytes and generates ions from the analyte, which are then directed for analysis using a mass spectrometer.
US Patent No. 6,803,205. Methods of measuring enzyme activity using peelable and resealable devices. Inventors: David Duffy, Gregory Kirk, Stewart Campbell, Olivier Schueller, Melina Agosto. Assignee: Surface Logix.
In this invention, devices and methods for performing assays on materials, particularly biological materials, are provided. The devices and methods make use of self-sealing members which can be applied to a flat surface to form wells to facilitate immobilization of materials on the flat surface. The devices can then be removed to yield a flat surface that facilitates the performance of processes on the immobilized material, or the detection of material.
US Patent No. 6,800,449. High throughput functional proteomics. Inventors: Paul Haynes, Nancy Andon. Assignee: Syngena Participations.
This invention provides a method of identifying proteins with a shared function from a protein pool. The method calls for the preparation of a protein pool. Then the pool is applied to a functional affinity column where the column isolates protein with a common function based on the affinity chromatographic behavior of the proteins. The isolated proteins are analyzed using a one or more dimensional column in combination with mass spectrometry to produce spectral information, which is matched with a theoretical mass spectrum for a protein having a known sequence. This is a high-throughput method for functional proteomics.
US Patent No. 6,800,453. Nucleic-acid programmable protein arrays. Inventors: Joshua Labaer, Albert Lau. Assignee: President and Fellow of Harvard College.
With this invention, arrays of polypeptides can be generated by translation of nucleic acid sequences encoding the polypeptides at individual addresses on the array. This allows for the rapid and versatile development of a polypeptide microarray platform for analyzing and manipulating biological information.
US Patent NO. 6,808,683. Droplet dispensing system. Inventor: John Gilbert. Assignee: Cytonome.
This invention provides a droplet dispensing system for forming and dispensing droplets of a liquid sample. The system is fabricated on the tip of a dispensing pin. The tip of the dispensing pin includes a droplet ejection nozzle, a filling channel, a chamber, and an actuator for ejecting droplets on demand.