US Patent No. 6,670,194. Rapid quantitative analysis of proteins or protein function in complex mixtures. Inventors: Rudolf Aebersold, Michael Gelb, Steven Gygi, Ronald Scott, Frantisek Turacek, Scott Gerber, Beate Rist. Assignee: University of Washington.
Covers reagents and methods for using affinity-labeled protein reactive reagents for the selective isolation of peptide fragments or reaction products from a mixture. These peptides are then analyzed by mass spectrometry. The reagents also can isotopically label the peptides or products for quantitative mass spectrometric analysis.
The method can be applied for qualitative and quantitative analysis of global protein expression profiles in cells and tissues and to screen for differential expression of proteins under different conditions. The reagents consist of an affinity label that may be isotopically labeled and covalently linked to a protein reactive group.
US Patent No. 6,661,520. Sensor system of surface plasmon resonance and measuring method thereof. Inventors: Chu-Wann Lin, Chi-Yu Huang, Jyh-Perng Chiu, Ying-Tsuen Liou, Shuen-Chen Shiue, Te-Son Kuo, Long-Sun Huang, Pei-Zen Chang, Lung-Jieh Yang, Chau-Chung Wu, Shiming Lin, Chih-Kung Lee. Assignee: National Taiwan University.
Covers a surface plasmon resonance system and measuring method for analyzing a substance, with the object of providing a protein chip that can analyze biological molecules. The system includes an optical device for generating two light beams in succession, a sensor for generating plasmon waves in response to the light beams, and a measuring device for measuring the spectra of the first and second reflective signals and obtaining a value for calculating a reference value that can then be used to analyze the substance.
US Patent No. 6,670,609. Sample support plates for mass spectrometry with ionization by matrix-assisted laser desorption. Inventors: Jochen Franzen, Jens Rebettke. Assignee: Bruker Daltonik.
Covers the structure of a sample support plate for matrix-assisted laser desorption mass spectrometric analysis of biomolecules. The plate is flat, electrically conductive, and bonded to a base structure to form the dimensions of a mictrotiter plate without thermal distortion.
US Patent No. 6,670,606. Preparation of ion pulse for time-of-flight and for tandem time-of-flight mass analysis. Inventors: Anatoli Verentchikov, Jennifer Campbell. Assignee: PerSeptive Biosystems (now Applied Biosystems).
Covers the use of a segmented ion trap with collisional damping for improving the resolution and mass accuracy of single and tandem time-of-flight mass spectrometers. For a single stage spectrometers, ions are injected from a pulsed ion source into the trap, where they are trapped by an RF field. The ions are then cooled and ejected into the TOF. For tandem analysis, the ions are injected into a TOF analyzer to pre-select particular ions and then injected into the trap, where they are fragmented.
US Patent No. 6,660,149. Multichannel microscale system for high throughput preparative separation with comprehensive collection and analysis. Inventors: Barry Karger, Lev Kotler, Frantisek Foret, Marek Minarik, Karel Kleparnik. Assignee: Beckman Coulter.
Covers a modular multiple lane or capillary electrophoresis-based automated parallel sample separation and collection system. Fractions are collected in a multi-well collection plate, preferably made of a gel. The invention also covers an optional further on-line autonmated sample fraction analysis.
US Patent No. 6,664,104. Device incorporating a microfluidic chip for separating analyte from a sample. Inventors: Farzad Pourahmadi, William McMillan, Jesus Ching, Ronald Chang, Lee Christel, Gregory Kovacs, Allen Northrup, Kurt Petersen. Assignee: Cepheid.
Covers a cartridge containing a flow-through microfluidic chip for the separation of an analyte from a fluid sample. The chip contains an array of structures for capturing the analyte and later releasing it. Also included in the cartridge are channels and at least one controller for directing the flow of sample and elution fluid. Also covers an optional lysing region, waste chamber, and reaction or detection chambers.
US Patent No. 6,660,146. Method for electrophoretic focusing. Inventors: Percy Rhodes, Robert Snyder. Assignee: None.
Covers an apparatus and method for free-fluid electrophoresis achieving high resolution separation and analytical capability comparable to that obtained with capillary zone electrophoresis. The invention combines features of electrophoresis and isoelectric focusing useful for large-scale purifications and fractionations of samples. The device includes a separation chamber bounded by precision-pore insulated screens, many purge chambers, electrode chambers, and pump means.