US Patent No. 6,682,942. Microdevices for screening biomolecules. Inventors: Peter Wagner, Dana Ault-Riche, Steffen Nock, Christian Itin. Assignee: Zyomyx.
Covers methods for using miniaturized microfabricated devices with immobilized biomolecules on the surface to conduct multiplexed screening of biomolecular activity. The inventors suggest that the methods be used for the parallel screening of families of related proteins in drug development, functional proteomics, and clinical diagnostics applications. Biomolecules that can be immobilized include proteins, polypeptides, polynucleotides, polysaccharides, phospholipids, and unnatural polymers.
US Patent No. 6,680,203. Fourier transform mass spectrometry of complex biological samples. Inventors: Jean-Louis Dasseaux, Roger Newton, Thomas Rea, Charles Bisgaier, Michael Pape. Assignee: Esperion Therapeutics.
Covers methods for using FT-MS to screen complex biological samples containing both high-molecular weight molecules, such as proteins, and low-molecular weight molecules, such as peptides and metabolites. The methods allow the user to gain high information content, including the elucidation of differences between samples, and the identification of biologically active molecules present in the samples.
US Patent No. 6,677,114. Polypeptide fingerprinting methods and bioinformatics database system. Inventors: Luke Schneider, Michael Hall, Robert Petesch, Jeffrey Peterson. Assignee: Target Discovery.
Covers a set of methods, compositions, apparatus, and a data retrieval system for conducting proteomics experiments that are based on capillary electrophoresis and mass spectrometry. The invention provides a method for separating polypeptide species from a sample mixture using one or more capillary electrophoresis devices, and then sequencing or identifying polypeptides by mass spectroscopy. The invention also covers a computer-based database query and retrieval system that can apply datasets to such uses as diagnostics, therapeutics, drug discovery, and drug development. In addition, the invention covers the use of the described methods, appara-tuses, compositions, and database systems.
US Patent No. 6,675,104. Method for analyzing mass spectra. Inventors: Chris Paulse, Edward Gavin, Leonid Braginsky, William Rich, Eric Fung. Assignee: Ciphergen Biosystems.
Covers a method for analyzing mass spectra using a computer. The method includes entering mass spectra data sets from several samples into the computer and assigning each sample a class characterized by a particular biological status. As part of the invention, a classification model is formed, which discriminates between the classes in the class set.
US Patent No. 6,683,303. Ion trap mass spectrometer and spectrometry. Inventors: Kiyomi Yoshinari, Yoshiaki Kato, Tadao Mimura, Shinji Nagai. Assignee: Hitachi.
Covers an ion trap mass spectrometer and spectrometry that allows for the efficient dissociation of ions independent of ionic species without wasting time and without performing MS/MS. The invention lengthens a period for applying a CID voltage in accordance with a mass number or the characteristics of ions to be dissociated in order to optimize the voltage application period.
US Patent No. 6,683,302. Method and device for electrospray ionization. Inventors: Johan Eriksson. Assignee: Amersham Biosciences.
Covers an electrospray device and a method for heating the liquid in the device. The device consists of a liquid source, a mass analyzer, an inlet plate with orifice, a means for liquid inlet, a spray tip for emitting liquid, and a means for emitting microwaves between the tip and the mass analyzer.
US Patent No. 6,683,299. Time-of-flight mass spectrometer for monitoring of fast processes. Inventors: Katrin Fuhrer, Marc Gonin, Kent Gillig, Thomas Egan, Michael McCully, John Schultz. Assignee: Ionwerks.
Covers a time-of-flight mass spectrometer that uses an interleaved timing scheme and a position-sensitive detector to monitor fast processes. In one embodiment, an ion source repetitively generates ions and an ion extractor extracts the ions from the source for the TOF section. A timing controller tracks and controls the time of activation of the ion source and activates the extractor according to a predetermined sequence. This predetermined sequence can also include a time offset between the activation of the ion source and of the ion extractor. The kinetic energy of the ions can also be adjusted.
US Patent No. 6,680,476. Summed time-of-flight mass spectrometry utilizing thresholding to reduce noise. Inventors: August Hidalgo, Ken Poulton. Assignee: Agilent Technologies.
Covers a summed TOF-MS that has a filter for identifying outputs that are likely the result of noise rather than ions. The instrument stores data values at locations determined by a register that counts clock pulses. The filter generates an output measurement value corresponding to the ion measurements that come from the ion detector, setting the output value to a baseline value if it determines that the ion measurement is probably noise. An adder sums the data value specified by the register value and the output measurement value.
US Patent No. 6,674,070. On-line and off-line deposition of liquid samples for matrix assisted laser desorption ionization-time of flight mass spectroscopy. Inventors: Barry Karger, Frantisek Foret, Jan Preisler. Assignee: Northeastern University.
Covers an interface for continuous on-line liquid sample introduction directly to the TOF-MS, where the liquid sample includes a solid or liquid matrix. The samples and matrices can be used as in conventional MALDI. The solution of sample contains, for example, peptides and a matrix and is infused directly into the source chamber of a mass spectrometer, deposited on a moving sample holder, and desorbed. The method is useful for multiplexing and high-throughput MS analysis. Also covers an off-line deposition chamber.