US Patent No. 6,980,674. Reference database. Inventors: Norman Anderson, N. Leigh Anderson. Assignee: Large Scale Proteomics Corp.
This invention relates to a method and means for systematically studying proteins to provide data, and to enable the making of a catalog of proteins. The method enables the rapid provisional identification of proteins between and among samples. The provisional identification, which later can be confirmed, then can be relied on to develop further provisional identifications of proteins in the same or other samples. The method provides a protein reference standard, be it for an individual protein, a set of proteins, or a pattern of polypeptide spots appearing on a 2D gel. That sort of reference standard can be applied across organelles, tissues, organs, individuals, and so on. The method provides a reference point to distinguish manifestations arising from an abnormal state, such as a disease state. The protein index or database can be aligned with a chromosomal map or to a morbid gene map to reveal associations with a particular protein and with a particular disease, respectively.
US Patent No. 6,977,150. Process and compositions for peptide, protein and peptidomimetic synthesis. Inventors: Anthony Forster, Stephen Blacklow. Assignee: Anthony Forster.
The invention is a simplified, highly-purified translation system that offers new, potentially improved routes to all peptides, proteins, and peptidomimetics. The process does not require the addition of translation factors EF-P, W, W2 or rescue.
US Patent No. 6,974,526. Plastic microfluidics enabling two-dimensional protein separations in proteome analysis. Inventors: Cheng Sheng Lee, Don DeVoe. Assignee: Calibrant Biosystems, The University of Maryland.
The invention provides a microfluidic apparatus for performing 2D biomolecular separations. An isoelectric point-based separation is enabled in the first dimension, and a size-based separation is enabled in a second dimension.
US Patent No. 6,972,326. Labeling of immobilized proteins using dipyrrometheneboron difluoride dyes. Inventors: Richard Haugland, Karen Martin, Wayne Patton. Assignee: Molecular Probes.
The invention describes methods for labeling or detecting proteins and peptides immobilized on membranes and other solid supports, using fluorescent dipyrrometheneboron difluoride dyes.
US Patent No. 6,982,414. Method of mass spectrometry and a mass spectrometer. Inventors: Robert Bateman, James Langridge, Therese McKenna, Keith Richardson. Assignee: Micromass UK.
A method of mass spectrometry is disclosed wherein a gas collision cell is repeatedly switched between a fragmentation and a non-fragmentation mode.
US Patent No. 6,979,816. Multi-source ion funnel. Inventors: Keqi Tang, Mickhail Belov, Aleksey Tolmachev, Harold Udseth, Richard Smith. Assignee: Battelle Memorial Institute.
The invention relates to a method for introducing ions generated in a region of relatively high pressure into a region of relatively low pressure by providing at least two electrospray ion sources.