In Print: Recent Proteomics Papers of Note
Journal: Cancer Genomics & Proteomics, Sept. 9 [Epub ahead of print]
Title: Identification of markers associated with highly aggressive metastatic phenotypes using quantitative comparative proteomics.
Authors: Terp MG; Lund RR; Jensen ON; Leth-Larsen R; Ditzel HJ.
The authors used LC-MS/MS to compare the proteomes of three human breast cancer cell lines with different metastatic potentials, identifying 44 proteins that showed altered expression between the metastatic and non-metastatic lines.
Journal: PLoS One, Sept. 12 [Epub ahead of print]
Title: Novel effects of hormonal contraceptive use on the plasma proteome.
Authors: Josse AR; Garcia-Bailo B; Fischer K; El-Sohemy A.
The authors used multiple-reaction monitoring mass spec to measure 54 high-abundance proteins in 783 women, comparing expression between users and non-users of hormonal contraceptives, identifying 19 differentially expressed proteins involved in inflammation, innate immunity, coagulation, and blood pressure regulation.
Journal:Proteomics, Sept. 12
Title:Ten years of standardizing proteomic data: a report on the HUPO-PSI spring workshop: April 12-14th, 2012, San Diego, USA.
Authors:Orchard S; Binz PA; Borchers C; Gilson MK; Jones AR; Nicola G; Vizcaino JA; Deutsch EW; Hermjakob H.
The authors report on the 2012 HUPO-PSI spring workshop and particularly on the finalization of standards for capturing the output of mass spec techniques like selected-reaction monitoring and quantitative proteomics.
Journal: Molecular & Cellular Proteomics, Sept. 13 [Epub ahead of print]
Title: High-resolution mapping of linear antibody epitopes using ultrahigh-density peptide microarrays.
Authors: Buus S; Rockberg J; Forsstr Oumlm BO; Nilsson P; Uhlen M; Schafer-Nielsen C.
The authors used ultrahigh-density peptide microarrays featuring several hundred thousand different peptides per array to examine the specificity of a panel of polyclonal antibodies and obtain detailed descriptions of their specificities.
Journal:Methods, Sept. 13 [Epub ahead of print]
Title:Quantitative real-time PCR as a sensitive protein-protein interaction quantification method and a partial solution for non-accessible autoactivator and false-negative molecule analysis in the yeast two-hybrid system.
Authors:Maier RH; Maier CJ; Hintner H; Bauer JW; Onder K.
The authors present a method using detection and readout of mRNA expression instead of proteins or conventional Y2H genetic reporters to identify protein-protein interactions with a higher sensitivity than offered by conventional Y2H techniques.
Journal: Proteomics Clinical Applications, Sept. 24 [Epub ahead of print]
Title: Remarkable temporal stability of high-abundance human plasma proteins assessed by targeted mass spectrometry.
Authors: Randall SA; McKay MJ; Pascovici D; Mahon K; Horvath L; Clarke SJ; Molloy MP.
The authors quantified 31 plasma proteins in healthy volunteers over two time courses to determine between-subject and within-subject biological variance, finding that none of the measured proteins exhibited significant fluctuations over either time course, suggesting the suitability of plasma proteins as disease markers.
Journal: PLoS One, Sept. 25 [Epub ahead of print]
Title: Proteome analysis identified the PPARγ ligand 15d-PGJ2 as a novel drug inhibiting melanoma progression and interfering with tumor-stroma interaction.
Authors: Paulitschke V; Gruber S; Hofstätter E; Haudek-Prinz V; Klepeisz P; Schicher N; Jonak C; Petzelbauer P; Pehamberger H; Gerner C; Kunstfeld R.
The authors used shotgun proteomics along with 2D-gel electrophoresis and immunoprecipitation of A375 melanoma cells treated with 15d-PGJ2, finding evidence that the agent might modify Hsp-90 activity along with several chaperones.
Journal: Genome Biology, Sept. 28 [Epub ahead of print]
Title: Correlation of proteome-wide changes with social immunity behaviors provides insight into resistance to the parasitic mite, Varroa destructor, in the honey bee (Apis mellifera).
Authors: Parker R; Guarna MM; Melathopoulos AP; Moon KM; White R; Huxter E; Pernal SF; Foster LJ.
The authors characterized the proteome-wide changes in larval integument and adult antennae in honey bees, identifying several proteins predictive of hygienic behavior enabling bees to resist various diseases.
Journal: Nature Chemical Biology, Sept. 30 [Epub ahead of print]
Title: Systems-pharmacology dissection of a drug synergy in imatinib-resistant CML.
Authors: Winter GE; Rix U; Carlson SM; Gleixner KV; Grebien F; Gridling M; Müller AC; Breitwieser FP; Bilban M; Colinge J; Valent P; Bennett KL; White FM; Superti-Furga G.
The authors combined phosphoproteomics, transcriptomics, and chemical proteomics to investigate the mechanisms underlying the strong synergy between the kinase inhibitors danusertib and bosutinib affecting CML cells harboring BCR-ABL.