While LC-MS/MS workflows using triple quadrupoles and, more recently, Q-TOF and Q-Exactive instruments, have typically dominated discussion of mass spec-based protein quantitation, MALDI mass spec has recently emerged as a potential alternative for such applications.

New sample prep techniques and forthcoming instrumentation should continue to drive the technology onward, said Mark Stolowitz, director of the Proteomic Core Facility at Stanford’s Canary Center for Cancer Early Detection and a MALDI mass spec expert.

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