Recent NSF Awards in Proteomics and Protein Research
Title: Electronic Allergy Diagnostics: Photo-Immobilization as a General Strategy for Attaching Structurally and Compositionally Diverse Ligands onto a Single Support
Principal Investigator: Brian Clare
Start/End Date: July 1, 2010 – Dec. 31, 2010
Amount Awarded to Date: $149,993
The project will look at the feasibility of using photochemical linkers to immobilize small molecules, synthetic peptides, purified proteins, and crude natural extracts on electrochemical sensor arrays. The research seeks to develop a single photochemical protocol for preparing the various assays, enabling the manufacture of highly multiplexed sensor arrays for use in point-of-care diagnostics.
Title: Nanoscopic Understanding of Protein Transport and Structure Dynamics in Charged Gels for Protein Chromatography
Principal Investigator: Giorgio Carta, Michael Shirts
Sponsor: University of Virginia Main Campus
Start/End Date: Aug. 15, 2010 – July 31, 2013
Amount Awarded to Date: $303,000
Funds a study to investigate the movement of proteins in charged gels for the development of new matrices for protein chromatography. The research will look at the structure and dynamic behavior of dextran-grafted matrices and the movement of individual proteins in these matrices at the nanoscopic scale.
Title: Surface-Initiated Enzymatic Polymerization of DNA Nanostructures for Highly Amplified Sensing
Principal Investigator: Ashutosh Chilkoti, Stefan Zauscher
Sponsor: Duke University
Start/End Date: Sept. 1, 2010 – Aug. 31, 2013
Amount Awarded to Date: $360,000
Funds research to use surface-initiated enzymatic polymerization, or SIEP, of DNA to develop a new on-chip signal detection and amplification system that is broadly applicable to a variety of multiplexed molecular detection schemes, including on-chip sensors, heterogeneous immunoassays, and protein microarrays.
Title: Large Scale Microtubule-Based Nanomanufacturing of Single Kinesin Patterns with Ultrahigh Resolution
Principal Investigator: Douglas Chrisey, Zoica Cerasela Dinu
Sponsor: Rensselaer Polytechnic Institute, West Virginia University Research Corporation
Start/End Date: Sept. 1, 2010 – Aug. 31, 2011
Amount Awarded to Date: $50,000
The project seeks to develop single protein nanoarrays based on self-assembly and self-recognition for use in areas such as nanosensors, screening, drug delivery, and as a proof of principle that biological molecules can be used for printing ultrahigh-resolution nanoarrays.