By Adam Bonislawski

Uppsala University scientists have developed an expanded proximity ligation assay that employs up to five antibodies to confirm the simultaneous presence of multiple proteins on a given target.

The researchers used the technique to isolate prostasomes — microvesicles secreted by prostate acinar cells that could be useful as prostate cancer biomarkers — but it could have wider applicability as a tool for top-down proteomics investigations into protein complexes and isoforms.

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