Comparison of Trypsin Brands Suggests Inexpensive Varieties Still Suitable for Proteomics Research | GenomeWeb

As the protease most commonly used to digest proteins into peptides for mass spec analysis, trypsin is a key part of most proteomic workflows, with the performance of the enzyme potentially affecting a variety experimental outcomes including sequence coverage and reproducibility.

A number of vendors sell trypsin, with their offerings ranging significantly in price. For instance, porcine trypsin from Promega – generally considered the gold standard within the field – costs $0.41 per μg. Bovine trypsin from Sigma-Aldrich, meanwhile, runs less than $0.01 per μg.

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