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ABRF's Protein Sequencing, Glycoprotein Groups Makes Test Samples Available


This story originally ran on Sept. 14 and has been updated to include an additional test sample that was made available.

The Association of Biomolecular Resource Facilities' Protein Sequencing Research Group and Glycoprotein Research Group have announced the availability of test samples for their 2010 studies.

PSRG said this year's study will investigate traditional and alternative methods of obtaining N-terminal information on a complex mixture. Participants will receive a monoclonal antibody, from which they will be asked to obtain as much N-terminal sequence of both light and heavy chains as possible.

Participants may use any available Edman, mass spectrometric, or biochemical method or technology, or a combination of techniques. PSRG wants to compare the extent of N-terminal sequence obtained by a variety of methods and is encouraging all mass spectrometry and proteomic labs as well as Edman sequencing facilities to participate.

PSRG was previously called the Edman Sequencing Research Group.

To obtain a sample and participate in the study, an e-mail should be sent to [email protected]. Final sample requests should be made by Sept. 25. Data should be submitted by Dec. 1.

Participants will be asked to submit their data electronically.

Also, gPRG said the objective of its study this year is to investigate methods of quantitatively identifying changes in a glycoprotein's glycan. It seeks to document the different approaches used by the ABRF community and to highlight the type of information obtained from these experiments.

In announcing the availability of the test samples, gPRF said that it anticipates the sample can be characterized by numerous glycoanalytical techniques such as ESI or MALDI of labeled or unlabeled glycans, and NP HPLC or RP HPLC of fluorescently labeled glycans.

Participants will be asked to identify the major N-linked glycans detected in three similar glycoprotein samples; determine any differences in the distribution of the N-linked glycans in the three mixtures; and provide a one- to two-page description of the methods they used for their analysis.

Results from the study and descriptions of the methods used will be compiled electronically, and methods that were successful in the determination of known differences in the sample sets will be highlighted.

Requests for samples must be submitted by Sept. 30 by e-mail to [email protected] The subject line should indicate that a sample for the gPRG2010 Quantitative Glycoprotein Sample is being requested.

gPRG expects samples to be distributed next month. Data should be returned by Dec. 15.

If a participant's facility restricts the type of biological samples it can receive, a participant should include the text "more information" in the subject line of the request. gPRF will send additional information about the nature of the study sample in order to hasten delivery.

Results from both studies will be presented at the ABRF 2010 annual meeting in March.

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