This story originally ran on Sept. 23.
The Association of Biomolecular Resource Facilities' Proteomics Research Group earlier this month made available its test samples for its 2010 study.
The study, whose results will be presented at ABRF's annual meeting in March, will explore the use of different approaches for "determining sample composition and identifying unforeseen problems in otherwise 'straight-forward' analyses," the group said in its announcement.
According to PRG the confirmation of protein identity and determination of qualitative differences among samples is a common experiment in proteomics laboratories. The experiments often involve proteins that are expressed exogenously for subsequent enzymatic and/or structural assays, and the presence of cloning tags, background proteins associated with affinity enrichment, and use of stable isotopic labeling strategies may create issues with the data.
The primary goal of the PRG study is to "document the breadth of approaches used by the ABRF community and highlight the type of information obtained," it said. The study is open to members and non-members of ABRF, but because of the limited supply of samples, priority will be given to ABRF members.
The study will involve several levels of experimental challenges to allow scientists with varying levels of experience and expertise to assess their abilities; submission of results in an online survey format; and comparison of best practices among respondents.
The PRG will compile descriptions of the experimental methods used and highlight those that successfully characterized the samples, including known differences in the sample sets.
Requests for samples should be e-mailed to [email protected] before Oct. 15. The words "PRG sample request" should be in the subject line. PRG expects to send out the samples in mid-October. Data should be returned by Dec. 15.