Life Technologies' Applied Biosystems said this week it is launching its TaqMan Protein Expression Assays in August.
The real-time PCR assays enable researchers to rapidly detect proteins and "to correlate relative levels of specific proteins with cell functions and behaviors, such as different disease conditions, states of pluripotency, or differentiation in stem cells," ABI said in a statement. The new assays are based on technology combining an antibody-oligonucleotide-tagged immunoassay with a TaqMan assay to generate real-time PCR data for proteins present in as little as 10 to 250 cells.
The initial launch will consist of six pre-designed protein expression assays, four directed at stem cell pluripotency markers, and two directed at "more commonly expressed proteins in a variety of cell types," the company said.
The National Center for Biotechnology Information at the National Institutes of Health announced the availability of the Peptidome database.
Peptidome archives and freely distributes tandem mass-spectrometry peptide and protein identification data generated by researchers. Its core structure is based on NCBI's Gene Expression Omnibus "and uses much of the same source code for handling submission and accessing the database," members of NCBI said in a letter to the editor of Nature Biotechnology announcing Peptidome's availability.
Like GEO, Peptidome was created to encourage data-sharing at a level that is useful for both a specialized community, as well as the general biological community. Emphasis was placed on simplifying the deposit procedures while still supporting a high level of experimental annotation, the NCBI officials said.
A Peptidome submission includes biological and methodological data; original raw spectra data; converted to open format; peptide identification output files; and conclusion-level results.
The database is available here.