VCU Team Marries AFM and qPCR for Highly Multiplexed Biomarker Assays | GenomeWeb

NEW YORK (GenomeWeb) — Researchers from Virginia Commonwealth University have developed a method of quantifying multiple nucleic acid targets in a single sample by combining low-cycle-number, target-specific PCR with atomic force microscopy.

The technique, according to its developers, has the potential to absolutely quantify target nucleic acids much in the same way that digital PCR does, but without the use of relatively expensive microfluidic platforms. Instead, the method makes use of standard laboratory equipment such as thermal cyclers and AFMs.

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