University of Rochester Group Develops Open Source Algorithm for qPCR Non-detects | GenomeWeb

NEW YORK (GenomeWeb) — Sometimes qPCR data is simply missing. It is standard practice in data processing to replace "non-detects," or reactions failing to produce a minimum amount of signal, with a cycle threshold value of 40. A study published last week in Bioinformatics, however, showed that this practice likely introduces bias and spurious interpretations, and proposed a new method.

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