Carl Wittwer, the University of Utah scientist who helped invent 15-minute PCR and many other commonly used PCR techniques and instrumentation, is back at the drawing board, whittling the time it takes to perform PCR to less than 30 seconds.

By employing high surface area-to-volume capillary tubes and up to 25-fold higher primer and polymerase concentrations than standard PCR, Wittwer and colleagues have used the method to successfully amplify a 49-bp fragment of human genomic DNA in less than 30 seconds and 102-bp fragments in less than a minute.

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Jul
14
Sponsored by
Agilent Technologies

This online seminar will outline a recent example of the use of molecular barcoding in combination with next-generation sequencing to detect somatic mosaicism in cancer patients.