NEW YORK (GenomeWeb) — To measure HIV viral load, researchers at Rice University in Houston have developed a technique that takes isothermal recombinase polymerase amplification, or RPA, to the next level.

The method, described as real-time, quantitative RPA, or qRPA, was published last month in Analytical Chemistry. It generates a single standard curve using internal control DNA, then uses an algorithm to analyze real-time fluorescence data and quantify HIV-1 DNA in samples.

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