qPCR's Big Bag of Tricks

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The sweeping rationale behind the increased use of quantitative PCR in the clinic — that almost any classically trained lab technician can perform the assay in any standard molecular biology lab — might also be the technique's biggest downfall in establishing itself as a reputable diagnostic. While qPCR is now commonly used to detect infectious disease pathogens for diagnostic purposes, serious doubts remain as to whether it will ever replace conventional culturing or standby sequencing techniques.

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