Illumina has launched its Nextera Exome and Custom Enrichment Kits for sample preparation for next-gen sequencing.
The kits require 50 nanograms of starting DNA. The exome kit targets more than 62 megabases of coding regions and untranslated regions, and provides 12-plex pre-enrichment sample pooling. Users can fully customize content using Illumina's DesignStudio, a free online tool.
Promega has launched its PowerPlex Y23 System, a rapid human identification Y-short tandem repeat assay for forensic casework, offender databasing, and relationship testing.
According to Promega, the new system cuts thermal cycling time roughly in half, and detects substantially more Y-STR loci than current systems. The multiplex system uses rapid cycling to co-amplify 23 Y-STR loci, which include many of the Y-STR loci found in key STR databases in the US and Europe.
The system works with extracted DNA, including difficult samples with lower amounts of template and male/female mixtures. Promega also provides streamlined protocols for reference, database, and paternity testing. The combination of reduced sample preparation time and shorter cycling times results in a reduction in total time to process samples — more than a half day per plate in many cases, Promega said.
The company added that a majority of early testers of the system were able to detect profiles with as little as 32.5 picograms of DNA, and detect complete profiles with 62 picograms of male DNA in the presence of 400 nanograms of female DNA (a 6,450-fold excess).