Fluidigm this week introduced the mRNA sequencing application for its C1 Single-Cell Auto Prep System.
The new application enables transcriptome analysis of 96 single cells to rapidly study differential expression profiles of diverse cell populations. The C1 mRNA Seq kits and integrated fluidic circuits enable the processing of up to 96 single-cell cDNA libraries to quantify mRNA expression on Illumina sequencers.
The C1 system workflow requires as few as 200 cells, uses as little as 10 picograms per cell, and requires no reagent mixing, fragmentation, or purification. Researchers use Fluidigm's BioMark HD system to conduct routine quality control on the cDNA library before running it on an Illumina sequencer.
Fluidigm said that the protocol and reagent kits will be commercially available in January.