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Product Watch: Fluidigm, Agilent, Bioline, Boreal Genomics


Fluidigm this week launched two products related to next-generation sequencing: the Access Array Barcode Library for Life Technologies' Ion Torrent PGM sequencer and Access Array Target-Specific Primers for targeted resequencing.

The PGM barcode library allows multiplexing of up to 96 different samples in a single sequencing run, the company said. It uses a bidirectional amplicon tagging approach to allow reads to be obtained from both ends and across the full length of the amplicon, which "eliminates the need for paired-end read sequencing,” Fluidigm CEO Gajus Worthington said in a statement.

The Access Array Target-Specific Primers, meantime, offered as part of a new custom assay design service for high-throughput Illumina platforms, allow up to 480 amplicons per sample for targeted resequencing, the company said.

Fluidigm claims the primers allow it to increase the number of amplicons per sample by 10-fold compared to its previous products.

Agilent Technologies has added two new products to its SureSelect line of sequencing target-enrichment products.

The HaloPlex target enrichment system for desktop sequencers "combines the speed and specificity of polymerase chain-reaction systems with the scalability and capture-size flexibility of in-solution hybridization formats," the company said.

The single-tube protocol requires no library prep and can reduce sample prep time to less than one day, Agilent said. The system is compatible with Illumina MiSeq desktop sequencers as well as HiSeq and GAIIx sequencers and is available under early access for Life Technologies' Ion Torrent platforms.

HaloPlex was developed by Uppsala, Sweden-based Halo Genomics, which Agilent acquired in December (PCR Insider 12/1/2011).

Agilent also launched the SureSelect XT Human Methyl-Seq system for DNA methylation sequencing.

The in-solution tool was developed to analyze under- and over-methylated cytosine sites on the human genome. The assay combines the company's SureSelect target-enrichment platform with bisulfite sequencing.

SureSelect XT Methyl-Seq allows researchers to analyze more than 3.7 million individual CpG dinucleotide sequences for their methylation state and targets promoters, canonical CpG islands, and the so-called “shores” and “shelves” found up to 4 kilobase pairs on either side of CpG islands.

Bioline, a wholly owned subsidiary of Meridian Bioscience, has released its SensiFast HRM Kit for high-resolution melt curve analysis.

The kit does not require labeled oligonucleotide probes, making it a cost-effective alternative to traditional probe-based genotyping methods, according to the company.

Boreal Genomics has launched an early-access program for its OnTarget Allele Enrichment System, which it plans to begin shipping in mid-2012.

OnTarget is based on the company's proprietary technology for the depletion of wild-type alleles. The company claims it offers single-nucleotide selectivity for improved detection of both known and unknown rare cancer mutations. Specifically, the firm said it can enrich rare cancer mutations at frequencies down to 0.01 percent from plasma, frozen, or formalin-fixed, paraffin-embedded tissues in less than two hours.

Boreal said it is currently working with researchers at Stanford University "to evaluate improvements to the detection of cancer mutations in heterogeneous tumor tissue where the majority of isolated DNA does not originate from the tumor."

The Scan

Genome Sequences Reveal Range Mutations in Induced Pluripotent Stem Cells

Researchers in Nature Genetics detect somatic mutation variation across iPSCs generated from blood or skin fibroblast cell sources, along with selection for BCOR gene mutations.

Researchers Reprogram Plant Roots With Synthetic Genetic Circuit Strategy

Root gene expression was altered with the help of genetic circuits built around a series of synthetic transcriptional regulators in the Nicotiana benthamiana plant in a Science paper.

Infectious Disease Tracking Study Compares Genome Sequencing Approaches

Researchers in BMC Genomics see advantages for capture-based Illumina sequencing and amplicon-based sequencing on the Nanopore instrument, depending on the situation or samples available.

LINE-1 Linked to Premature Aging Conditions

Researchers report in Science Translational Medicine that the accumulation of LINE-1 RNA contributes to premature aging conditions and that symptoms can be improved by targeting them.