Skip to main content
Premium Trial:

Request an Annual Quote

Product Watch: Empire Bioscience's FlashTaq Hot-Start Polymerase

Premium

Empirical Bioscience this week introduced FlashTaq Hot-Start Taq DNA polymerase, a chemically-modified Taq polymerase designed to reduce non-specific amplification in PCR.

The enzyme offers fewer contamination issues and fast activation time, according to the company. Under standard hot start conditions the enzyme can be activated and it recovers both 5'-3' polymerase and 5'-exonuclease activities within two minutes versus the ten minutes of activation time required by many competing enzymes.

"Unlike traditional non-hot start Taq, which can become active at room temperature and begin to amplify non-specifically and form primer-dimers, FlashTaq will not become active until the temperature and time threshold has been reached, thus reducing the likelihood of non-specific amplification and primer-dimer formation," Pushpaja Dodla a molecular biologist with Empirical Bioscience, said in a statement.

In addition to its stability and rapid activation time, other factors help FlashTaq streamline studies, including the type of modifying reagent that it uses, Empire said. The enzyme in FlashTaq has a reduced risk of contamination because it has been chemically as opposed to antibody-modified. Enzymes that are antibody-modified are highly susceptible to contamination from the organism in which they are produced, the company said.

Filed under

The Scan

Myotonic Dystrophy Repeat Detected in Family Genome Sequencing Analysis

While sequencing individuals from a multi-generation family, researchers identified a myotonic dystrophy type 2-related short tandem repeat in the European Journal of Human Genetics.

TB Resistance Insights Gleaned From Genome Sequence, Antimicrobial Response Assays

Researchers in PLOS Biology explore M. tuberculosis resistance with a combination of sequencing and assays looking at the minimum inhibitory concentrations of 13 drugs.

Mendelian Disease Genes Prioritized Using Tissue-Specific Expression Clues

Mendelian gene candidates could be flagged for further functional analyses based on tissue-specific transcriptome and proteome profiles, a new Journal of Human Genetics paper says.

Single-Cell Sequencing Points to Embryo Mosaicism

Mosaicism may affect preimplantation genetic tests for aneuploidy, a single-cell sequencing-based analysis of almost three dozen embryos in PLOS Genetics finds.