DNA Software has launched a free beta version of qPCR CopyCount, a cloud-based tool for providing absolute DNA copy number in qPCR experiments without calibration standards or special instrumentation.
DNA Software announced the product last week at Cambridge Healthcare Institute's inaugural digital PCR conference in San Diego.
The company is marketing qPCR CopyCount as a way to achieve the absolute quantification that can be obtained using digital PCR, but without the purchase of specialized instrumentation. Users need only upload their qPCR data obtained using standard real-time PCR instrumentation to obtain absolute DNA copy number.
The method requires no quantification standards and no instrument calibrations; can work with all widely used fluorophores; and accounts for PCR efficiency and provides an error rate of less than 4 percent, according to the company.
The method also accounts for decreasing PCR efficiency that occurs with each cycle of PCR and that varies for different assays, and can analyze a user's back library of previous results to produce absolute copy number for each well, allowing for the meta-analysis of data, DNA Software said.
Bio-Rad has launched its semi-automated PX1 PCR plate sealer and heat seals.
Designed for microplate sealing in PCR, real-time PCR, and Droplet Digital PCR applications, the PX1 PCR plate sealer and films and foils remove human variability from plate sealing and prevent sample evaporation from impacting results, Bio-Rad said.
Other benefits of the system include fast startup time, with the platform ready to seal in less than three minutes; touch-screen interface; and small benchtop footprint, the company said.
HTG Molecular Diagnostics this week unveiled the EDGE System at the Association for Molecular Pathology meeting in Long Beach, Calif.
The EDGE system is a fully automated platform that is designed for clinical and research laboratories and offers nucleic acid extraction-free gene expression results from formalin-fixed, paraffin-embedded tissue and other sample types.
Developed under ISO 13485:2003 standards, the EDGE system's barcode-driven template or custom user select protocols allow multiplexing of up to 47 genes per well using less than a single 5 µm section of FFPE tissue. It eliminates the time-consuming and complex qPCR processes of extraction, amplification, and labeling and can deliver up to 1,500 answers per 24-hour cycle, HTG said.