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Product Watch: Bio-Rad Supermixes; Enzymatics DNA Sample Prep; Roche Target Enrichment Kit


Bio-Rad Laboratories this week launched its SsoAdvanced universal supermixes, designed to deliver superior real-time PCR performance under any reaction conditions on any qPCR instrument.

The new SsoAdvanced universal supermixes include the SsoAdvanced universal SYBR Green supermix and the SsoAdvanced universal probes supermix. Both supermixes include Bio-Rad's patented Sso7d fusion enzyme, which the company claims provides the highest-quality data from the most challenging sample types. The enzyme's performance is aided by an advanced formulation of surfactants, detergents, stabilizers, and other proprietary components, allowing the supermixes to be used across a wide range of cycling conditions, primer concentrations, and temperatures.

The supermixes are compatible with all qPCR platforms. Multiple passive reference dyes blended in Bio-Rad's supermixes are particularly useful for researchers using qPCR instruments that require ROX fluorescent dyes, the company said.

Enzymatics this week launched the Spark DNA sample prep kits for Illumina and Ion Torrent sequencing platforms.

The new kits incorporate Enzymatics' proprietary room temperature-stable library technology and exceed performance of existing liquid-format library prep kits by reducing reagent pipetting by 30 percent, Enyzmatics said.

The kits can also be shipped and stored at ambient temperatures, obviating the cold chain and dramatically reducing storage, logistics, and validation costs, the company said.

Roche this week announced the global launch of its SeqCap EZ Pure Capture Bead Kit supporting target enrichment workflows prior to DNA sequencing.

The new kit is designed to provide sensitive and robust methods for capture and purification of DNA sample libraries enabling high-quality targeted sequencing results. With the addition of the kit to its capture portfolio, Roche now offers SeqCap EZ Library probe pools and all target enrichment reagents in a one-stop shop, the company said.

The Scan

UK Team Presents Genetic, Epigenetic Sequencing Method

Using enzymatic DNA preparation steps, researchers in Nature Biotechnology develop a strategy for sequencing DNA, along with 5-methylcytosine and 5-hydroxymethylcytosine, on existing sequencers.

DNA Biobank Developed for French Kidney Donors, Recipients

The KiT-GENIE biobank described in the European Journal of Human Genetics contains DNA samples, genotyping profiles, immune patterns, and clinical features for thousands of kidney donors or transplant recipients in Nantes, France.

Cardiometabolic Disease May Have Distinct Associations With Microbial Metabolites in Blood, Gut

By analyzing gut microbes in combination with related metabolites in feces and blood, researchers in Nature Communications found distinct cardiometabolic disease relationships at each site.

Study Reveals New Details About Genetics of Major Cause of Female Infertility

Researchers in Nature Medicine conducted a whole-exome sequencing study of mote than a thousand patients with premature ovarian insufficiency.