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Product Watch: Bio-Rad, Sedia Biosciences

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Bio-Rad Laboratories last week announced the launch of the SingleShot family of cell lysis RT-qPCR kits.

The new kits allow researchers to obtain RT-qPCR data directly from cultured cells without the need for a separate RNA purification step, and they provide high-quality gene expression results in less than two hours, Bio-Rad said.

The kits feature minimal setup and pipetting steps and thus are automation-friendly, and are the only available kits that include an RNA control template and qPCR assay to help researchers determine optimal cell number and lysate inputs for their RT-qPCR reactions, Bio-Rad said.

The SingleShot kits are validated for use with a wide variety of adherent and suspension cell lines and are offered in multiple formats, including one-step RT-qPCR and two-step RT-qPCR kits that are compatible with either SYBR Green or probe-based assays. SingleShot kits are also available as a stand-alone cell lysis kit, and are validated for use with the company's PrimePCR assays and panels.


Sedia Biosciences has launched the Asanté DNA Specimen Collection kit and Asanté DNA Purification Kit.

The Asanté DNA Specimen Collection Kit is intended as a cost-competitive alternative to current methods for DNA specimen collection, and is characterized by low DNA retention, minimal interfering substances, and ease of use. The kit provides samples that are suitable for testing by most in vitro DNA amplification methods including PCR and variations thereof, helicase-dependent amplification, ligase chain reaction, loop-mediated isothermal amplification, and others.

Meantime, the Asanté DNA Purification Kit is intended to facilitate extraction, purification, and concentration of DNA from the specimen collection kit. This product was originally designed for collection of oral DNA specimens with minimal downstream processing or extraneous reagent introduction while maximizing DNA yield. Researchers that have evaluated the product during development have also found that it is useful for collecting DNA from a variety of tissue types and locations, including genitourinary tract specimens, cadaverous samples, tissue samples for speciation and identification, and food samples for pathogen testing.

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