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Product Watch: Bio-Rad PrimePCR Rat Genome Assays; LGC KASP Genotyping Assays


Bio-Rad this week announced the launch of PrimePCR Rat Genome Assays for qPCR.

The new assays expand Bio-Rad's line of wet-lab-validated assays that currently include human and mouse genome assays. They are fully validated for specificity, efficiency, and sensitivity, and help researchers adhere to the minimum information for publication of quantitative real-time PCR experiments (MIQE) guidelines. As part of this validation process, Bio-Rad validates all PCR products using next-generation sequencing, verifying the percentage of on-target amplification. In addition, all of the validation raw data are available to the customer.

Researchers will be able to order PrimePCR Rat Genome Assays as individual assays (desalted or HPLC-purified), or build a custom plate (96- or 384-well). Assays are available for more than 20,000 rat genes as SYBR- and probe-based assays with a choice of five different fluorophores, the company said.

LGC said this week that it has launched 110,000 new wet-lab-validated KASP genotyping assays, available exclusively through the company's new online KASP assay search tool.

In the past decade LGC has run more than a billion KASP genotyping PCR reactions in its service labs across the world, the company said. A central part of this work has been validating the assays, which target hundreds of thousands of SNPs and other genetic variations associated with human diseases, the company added.

The new tool "allows easy searching and ordering of KASP genotyping assays that help scientists to further understand a wide variety of disease areas including cancer, cardiovascular, respiratory, and infectious," Joris Parmentier, LGC's product manager for genomics, said in a statement. He added that the company plans to add new batches of assays over time, "covering not just genetic variation in humans but variation in plant and animal genomes."

The assays can be run on most qPCR instruments, LGC said.