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Product Watch: Bio-Rad PrimePCR Assays; Bioline MyTaq Extract-PCR Kit


Bio-Rad has launched PrimePCR assays for Droplet Digital PCR.

The new pre-designed assays for mutation detection and copy number variation have been experimentally validated to provide single-copy resolution without a standard curve, Bio-Rad said. In this first release, Bio-Rad is offering 14 mutation-detection assays with matching wild-type assays for biologically relevant and highly prevalent cancer mutation targets, such as BRAF V600E and EGFR T790M, chosen from the Catalogue of Somatic Mutations in Cancer, or COSMIC, database.

Combined with Bio-Rad's QX100 Droplet Digital PCR system, the assays can detect a single mutant copy in a background of 2,000 or more wild-type molecules (0.05 percent mutation frequency). In addition, researchers can use 62 assays targeting common cancer genes and two reference target assays to discriminate small-fold copy number changes.

Bio-Rad said it has partnered with BioGazelle and Integrated DNA Technologies to design, optimize, and experimentally validate the assays. The assays use universal cycling conditions and are available in multiple reaction sizes.

Bioline, a subsidiary of Meridian Bioscience, has launched the MyTaq Extract-PCR kit.

Powered by the high-performance MyTaq DNA Polymerase, the new kit offers a quick and easy alternative for extracting and amplifyinfg DNA from a variety of mammalian tissue types, Bioline said. Researchers using the kit can replace complicated and time-consuming extraction methods with a 15-minute, single-tube protocol, making it ideal for high-throughput applications such as mouse genotyping and sequencing, the company said.

With the new kit, researchers can now "quickly achieve the purity and yield necessary for high-performance PCR and real-time PCR experiments from solid tissues, such as mouse tail or mouse ear," Bioline President Marco Calzavara said in a statement.

The Scan

US Booster Eligibility Decision

The US CDC director recommends that people at high risk of developing COVID-19 due to their jobs also be eligible for COVID-19 boosters, in addition to those 65 years old and older or with underlying medical conditions.

Arizona Bill Before Judge

The Arizona Daily Star reports that a judge weighing whether a new Arizona law restricting abortion due to genetic conditions is a ban or a restriction.

Additional Genes

Wales is rolling out new genetic testing service for cancer patients, according to BBC News.

Science Papers Examine State of Human Genomic Research, Single-Cell Protein Quantification

In Science this week: a number of editorials and policy reports discuss advances in human genomic research, and more.