Skip to main content
Premium Trial:

Request an Annual Quote

Product Watch: Bio-Rad PrimePCR Assays; Bioline MyTaq Extract-PCR Kit

Premium

Bio-Rad has launched PrimePCR assays for Droplet Digital PCR.

The new pre-designed assays for mutation detection and copy number variation have been experimentally validated to provide single-copy resolution without a standard curve, Bio-Rad said. In this first release, Bio-Rad is offering 14 mutation-detection assays with matching wild-type assays for biologically relevant and highly prevalent cancer mutation targets, such as BRAF V600E and EGFR T790M, chosen from the Catalogue of Somatic Mutations in Cancer, or COSMIC, database.

Combined with Bio-Rad's QX100 Droplet Digital PCR system, the assays can detect a single mutant copy in a background of 2,000 or more wild-type molecules (0.05 percent mutation frequency). In addition, researchers can use 62 assays targeting common cancer genes and two reference target assays to discriminate small-fold copy number changes.

Bio-Rad said it has partnered with BioGazelle and Integrated DNA Technologies to design, optimize, and experimentally validate the assays. The assays use universal cycling conditions and are available in multiple reaction sizes.


Bioline, a subsidiary of Meridian Bioscience, has launched the MyTaq Extract-PCR kit.

Powered by the high-performance MyTaq DNA Polymerase, the new kit offers a quick and easy alternative for extracting and amplifyinfg DNA from a variety of mammalian tissue types, Bioline said. Researchers using the kit can replace complicated and time-consuming extraction methods with a 15-minute, single-tube protocol, making it ideal for high-throughput applications such as mouse genotyping and sequencing, the company said.

With the new kit, researchers can now "quickly achieve the purity and yield necessary for high-performance PCR and real-time PCR experiments from solid tissues, such as mouse tail or mouse ear," Bioline President Marco Calzavara said in a statement.

The Scan

Germline-Targeting HIV Vaccine Shows Promise in Phase I Trial

A National Institutes of Health-led team reports in Science that a broadly neutralizing antibody HIV vaccine induced bnAb precursors in 97 percent of those given the vaccine.

Study Uncovers Genetic Mutation in Childhood Glaucoma

A study in the Journal of Clinical Investigation ties a heterozygous missense variant in thrombospondin 1 to childhood glaucoma.

Gene Co-Expression Database for Humans, Model Organisms Gets Update

GeneFriends has been updated to include gene and transcript co-expression networks based on RNA-seq data from 46,475 human and 34,322 mouse samples, a new paper in Nucleic Acids Research says.

New Study Investigates Genomics of Fanconi Anemia Repair Pathway in Cancer

A Rockefeller University team reports in Nature that FA repair deficiency leads to structural variants that can contribute to genomic instability.