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Product Watch: Bio-Rad Precision Blue qPCR Dye; Promega Maxwell RNA FFPE Kit


Bio-Rad this week launched its Precision Blue real-time PCR dye.

Designed to make it easier for researchers to set up qPCR experiments, the blue dye improves the visibility of qPCR reaction mixtures, enabling loading of small-volume reactions with greater speed, accuracy, and precision, Bio-Rad said.

Applications such as SNP genotyping and high-throughput gene expression analysis using 384-well plates will particularly benefit from use of this dye, the company noted.

Precision Blue is compatible with Bio-Rad's universal qPCR supermixes, including iTaq and SsoAdvanced, and does not interfere with SYBR Green- or probe-based detection. The dye can be used with all Bio-Rad CFX real-time PCR systems, as well as other vendors' real-time PCR systems.

Precision Blue can be added directly to supermix or used as a component of a reaction master mix. The dye is concentrated 200x, and delivers 500 20-µl reactions.

Promega has launched the Maxwell 16 LEV RNA FFPE kit for RNA isolation from mammalian formalin-fixed, paraffin-embedded tissue samples.

The new kit is designed to rapidly and consistently extract pure, high-yield RNA from thin sections of FFPE tissue samples using the automated Maxwell 16 instrument. The purified RNA is suitable for downstream applications including RT-PCR, RT-qPCR, endpoint RT-PCR, cDNA library synthesis, and gene expression analysis.

The system is specifically designed to purify RNA from 5-µm sections of FFPE samples, and can extract from 16 samples in a single run. Lysates are placed directly into the cartridges, and RNA is ready for amplification in approximately 60 minutes (after proteinase K digest). The kit does not use organic solvents such as xylene, thereby providing a much safer method than other available kits, and quality testing has shown virtually no PCR inhibitor contaminants are in purified samples.

The Scan

Interfering With Invasive Mussels

The Chicago Tribune reports that researchers are studying whether RNA interference- or CRISPR-based approaches can combat invasive freshwater mussels.

Participation Analysis

A new study finds that women tend to participate less at scientific meetings but that some changes can lead to increased involvement, the Guardian reports.

Right Whales' Decline

A research study plans to use genetic analysis to gain insight into population decline among North American right whales, according to CBC.

Science Papers Tie Rare Mutations to Short Stature, Immunodeficiency; Present Single-Cell Transcriptomics Map

In Science this week: pair of mutations in one gene uncovered in brothers with short stature and immunodeficiency, and more.