Skip to main content
Premium Trial:

Request an Annual Quote

Product Watch: Akonni TruTip and Illumina TruSeq Sample Prep Kits

Premium

Akonni Biosystems this week introduced a new family of TruTip extraction products for rapidly purifying human genomic DNA on the Hamilton Microlab STAR line of robotic liquid handlers.

Starting from fresh or frozen blood, buffy coat, and soon tissue or cells, the new TruTip kits extract superior quality gDNA simultaneously from eight to 96 samples in 57 minutes, Akonni said. The system does not require a magnetic head, vacuum manifold, centrifuge, or other space-consuming or expensive capital equipment. The procedure eliminates PCR inhibition due to bead carryover and errors due to clogging typically caused by vacuum manifolds.

The TruTip family of products uses a porous nucleic acid binding matrix embedded in a pipette tip, and extracts DNA or RNA using chaotropic salt chemistry. The kits can be used with a variety of downstream analytical tools, including qPCR, real-time PCR, digital PCR, microarrays, mid-multiplex bead-based systems, and traditional and next-generation sequencing systems.

TruTip genomic DNA Kits for use on the STAR line of workstations are now available for early-access customers. TruTip applications for extracting genomic DNA from saliva collected using the Oragene Discover and Oragene Dx Collection Kits from DNA Genotek, a subsidiary of OraSure Technologies, will be available soon, Akonni said.


Illumina has launched its TruSeq Stranded mRNA and Total RNA sequencing sample prep kits.

The kits enable RNA-seq studies from low-quality samples, such as formalin-fixed, paraffin-embedded samples, according to the company. The kits come either in 48-sample, low-throughput format containing 12 of 24 total available indexes; or 96-sample, high-throughput format.

The Scan

UK Pilot Study Suggests Digital Pathway May Expand BRCA Testing in Breast Cancer

A randomized pilot study in the Journal of Medical Genetics points to similar outcomes for breast cancer patients receiving germline BRCA testing through fully digital or partially digital testing pathways.

Survey Sees Genetic Literacy on the Rise, Though Further Education Needed

Survey participants appear to have higher genetic familiarity, knowledge, and skills compared to 2013, though 'room for improvement' remains, an AJHG paper finds.

Study Reveals Molecular, Clinical Features in Colorectal Cancer Cases Involving Multiple Primary Tumors

Researchers compare mismatch repair, microsatellite instability, and tumor mutation burden patterns in synchronous multiple- or single primary colorectal cancers.

FarGen Phase One Sequences Exomes of Nearly 500 From Faroe Islands

The analysis in the European Journal of Human Genetics finds few rare variants and limited geographic structure among Faroese individuals.