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Product Watch: Feb 24, 2010


Agilent Technologies this week announced the availability of Brilliant III Ultra-Fast qPCR and qRT-PCR Master Mix kits.

According to Agilent, the Brilliant III Ultra-Fast reagents are designed to provide the fastest cycling times on any real-time PCR instrument. The reagents feature a newly engineered Taq, delivering a faster extension rate combined with a novel hot-start technology to minimize non-specific amplification.

The ultra-fast reagents allow the completion of real-time experiments in less than 40 minutes without compromising the accuracy and reproducibility of nucleic acid quantification, the company said.

The reagents are validated across a range of real-time PCR systems and templates and targets. Earlier threshold cycle detection gives more reliable quantification across the entire range of template concentrations, including high and low concentrations.

The Brilliant III Master Mixes are Agilent's third generation of real-time PCR reagents to be commercialized, the company said.

Agilent also recently released a new applications bulletin that describes an automated system for rapidly characterizing pathogens, including the influenza A (H1N1) virus, on the company's BioCel PCR Viral Genotyping System.

Designed and built for researchers at Los Alamos National Laboratory and the University of California-Los Angeles, the BioCel PCR Viral Genotyping System provides rapid, reliable, and efficient automated genetic sequencing of viruses, Agilent said.

The system can automatically sequence 40 RNA samples across 192 genes in 11 hours. Using Agilent VWorks dynamic scheduling software, the system can sequence a minimum of 10,000 influenza viruses per year, Agilent said.

New England Biolabs said this week that it has expanded its line of sample preparation reagents to include NEBNext DNA Sample Prep Master Mix Set 3, which has been validated for use with the SOLiD 3 system from Life Technologies.

NEBNext reagents are a series of highly pure reagents that facilitate sample preparation for downstream applications such as sequencing, NE Biolabs said. Available as sets, master mixes, or modules, the reagents allow customization based on the sequencing platform being used.

Thermo Fisher Scientific this week announced the availability of the GENEius product search tool on the Thermo Scientific Dharmacon website.

The tool helps RNAi researchers select the most appropriate gene-specific products for their applications, such as gene silencing (siRNA and shRNA), microRNA (mimics and inhibitors), and qPCR detection (probe/primer assays), Thermo Fisher said.

To use the search, customers enter a gene identifier (e.g., a reference sequence accession, gene ID, gene symbol, gene description, Sanger ID, accession, or catalog number), and check their species of interest. The search tool then displays tabbed pages for RNAi, qPCR detection, transfection reagents, controls, and ancillary reagents.

The Scan

And Back

The New York Times reports that missing SARS-CoV-2 genome sequences are back in a different database.

Lacks Family Hires Attorney

A lawyer for the family of Henrietta Lacks plans to seek compensation from pharmaceutical companies that have used her cancer cells in product development, the Baltimore Sun reports.

For the Unknown

The Associated Press reports that family members are calling on the US military to use new DNA analysis techniques to identify unknown sailors and Marines who were on the USS Arizona.

PLOS Papers on Congenital Heart Disease, COVID-19 Infection Host MicroRNAs, Multiple Malformation Mutations

In PLOS this week: new genes linked to congenital heart disease, microRNAs with altered expression in COVID-19, and more.