Qiagen said this week that it has launched the Multiplex PCR Plus kit for rapid and sensitive PCR amplification of several targets in one run.
The Multiplex PCR Plus kit master mix includes HotStarTaq Plus DNA polymerase and a balanced combination of salts and additives to increase multiplex reaction specificity and ensure comparable efficiencies for all primers in the reaction, Qiagen said. The kit also includes CoralLoad buffer containing two dyes that improve pipetting visibility during reaction setup, gel loading, and DNA migration visualization.
The kit allows assay development for a variety of challenging applications, the company said, including mutation or microsatellite detection and microbial genotyping.
Promega said this week that its PowerPlex 18D system has received approval for use by labs generating DNA records for the National DNA Index System.
The NDIS is part of the Federal Bureau of Investigation's Combined DNA Index System. It contains several million offender and forensic DNA profiles contributed by federal, state, and local forensic labs used to identify criminals.
Promega launched the PowerPlex 18D system in February. The system is an 18-locus, multiplex STR amplification system designed for rapid DNA typing from offender database and familial relationship samples. According to the company, the system provides direct amplification, eliminating the need for a separate DNA extraction step, while providing a thermal cycling time of less than 90 minutes.
Genetic ID this week introduced the Fast ID 96-well extraction kit for isolating DNA from a variety of agricultural and food products.
The company said that the Fast ID 96-well kit is applicable to a wide range of simple and complex sample types, including seeds, plants, bacteria, animal tissue, and blood. The new 96-well format allows extraction in a higher throughput fashion than the company's previous kit. Extracted and purified genomic DNA is suitable for multiple downstream molecular biology applications, such as PCR amplification, restriction digestion, cloning, and sequencing, Genetic ID said.