Fluidigm this week introduced its 192.24 Dynamic Array Integrated Fluidic Circuit, designed to genotype 192 samples against 24 SNP assays in a single run.
Using the Fluidigm IFC Controller RX, the new Dynamic Array IFC enables sample loading in 30 minutes and thermal cycling in another 30 minutes, providing 4,608 data points in about an hour.
Despite the increased throughput, the new IFC retains the 99.75 percent or greater call accuracy and 99 percent or greater call rates associated with the company's other IFC products. The new chip can be read using either the BioMark HD or EP1 system.
Fluidigm said that the new IFC has immediate applicability in agricultural biotechnology and genome-wide association studies.
Fluoresentric has introduced Direct-2-PCR, Bact-2-PCR, and Hit-2-PCR, a trio of real-time PCR-dedicated sample preparation kits. Each kit is specifically designed to rapidly isolate DNA or RNA in buffers specifically formulated for real-time PCR detection, quantification, and genotyping.
Direct-2-PCR is a simple three-step protocol for isolating viral and/or genomic DNA and RNA from nasal or buccal swabs, and is ideal for genotyping, pathogen screening, forensics, and one- and two-step RT-PCR, Fluoresentric said. Direct-2-PCR sample prep can be completed in less than 10 minutes with less than two minutes of hands-on time per sample, the company added.
Meantime, Bact-2-PCR is designed to isolate bacterial DNA from any sample, including difficult-to-lyse organisms such as Mycobacteria; but is gentle enough to isolate DNA from even mammalian cell culture, the company said. Bact-2-PCR sample prep takes about 10 minutes for simple bacteria and about 35 minutes for anthrax and M. tuberculosis, using nothing more than a heating block and microfuge.
Hit-4-PCR is a real-time PCR-specific version of the commonly used 96-well filter plate DNA extraction systems. While other such systems use salts and alcohols that may be incompatible with real-time PCR, Hit-4-PCR replaces those chemistries with others better suited for the technique.
DNA Genotek this week launched OmniGene Discover, a new method to collect and stabilize microbial DNA for downstream molecular detection and analysis of pathogens.
OmniGene Discover provides long-term stability of microbial DNA at ambient temperature, facilitating storage and transport. The product is ideal for large-scale sample collections or for use in low-resource settings where the costs and logistics of cold chain storage and transportation are challenging, DNA Genotek said.