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Product Watch: Mar 24, 2011

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Qiagen this week launched the Investigator Quantiplex kit for quickly confirming that forensic DNA samples contain sufficient DNA for genetic fingerprinting analysis.

The kit, based on real-time PCR, quantifies human DNA in a sample and establishes whether it contains inhibitors that may interfere with downstream applications. The kit provides results in less than 50 minutes using the Rotor-Gene Q thermocycler; but it is also compatible with other instruments.

Qiagen said that results are ensured by using the appropriate target sequence and an integrated internal control that tests for successful amplification or PCR inhibition. The kit has an overall sensitivity of less than 1 pg/µL; and the procedure can be automated on the QIAgility system.


Integrated DNA Technologies said this week that it is now offering PrimeTime pre-designed qPCR assays for human, mouse, and rat transcriptomes.

According to IDT, assay users can search and select from an online database that contains thousands of assays, all of which are designed to span exons, protect against non-specific amplifications, avoid known SNPs, and minimize the impact of secondary structure.

The assays consist of two primers and a hydrolysis probe shipped in a single tube; and are guaranteed to provide greater than 90 percent efficiency, the company said.

The Scan

Researchers Compare WGS, Exome Sequencing-Based Mendelian Disease Diagnosis

Investigators find a diagnostic edge for whole-genome sequencing, while highlighting the cost advantages and improving diagnostic rate of exome sequencing in EJHG.

Researchers Retrace Key Mutations in Reassorted H1N1 Swine Flu Virus With Avian-Like Features

Mutations in the acidic polymerase-coding gene boost the pathogenicity and transmissibility of Eurasian avian-like H1N1 swine influenza viruses, a PNAS paper finds.

Genome Sequences Reveal Evolutionary History of South America's Canids

An analysis in PNAS of South American canid species' genomes offers a look at their evolutionary history, as well as their relationships and adaptations.

Lung Cancer Response to Checkpoint Inhibitors Reflected in Circulating Tumor DNA

In non-small cell lung cancer patients, researchers find in JCO Precision Oncology that survival benefits after immune checkpoint blockade coincide with a dip in ctDNA levels.