New England Biolabs this week expanded its line of PCR reagents by introducing OneTaq and OneTaq Hot Start DNA polymerases.
The new products are optimized blends of Taq and Deep Vent DNA polymerases, and offer robust amplification across a wide range of templates, including routine, AT-rich, and GC-rich amplicons, NEB said. The 3'-to-5' exonuclease activity of Deep Vent increases the fidelity and robustness of Taq, while the optimized buffers further enhance yield and product purity, regardless of a template's GC content, the company said.
In addition, OneTaq Hot Start DNA polymerase uses NEB's aptamer technology, which blocks polymerase activity at temperatures below 45°C, while the polymerase is activated during normal cycling conditions. This allows users to set up reactions at room temperature without a separate activation step, the company said.
NEB said that it is inviting researchers to sample OneTaq and OneTaq Hot Start and submit comments with data.
NorDiag this week said that it is now offering for sale to hospitals, laboratories, and research institutes a portfolio of sample preparation kits for the Nordag Arrow instrument for automated isolation of nucleic acids from all sample types.
The portfolio consists of three different applications, NorDiag said: Arrow RNA, Arrow DNA, and Arrow cell separation. NorDiag's Arrow instrument has the capacity to perform up to 10,000 nucleic acid isolations per year, the company said.
In addition, NorDiag has a portfolio of CE IVD marked sample preparation kits, such as DNA from stool, Viral NA, Bugs & Beads, and DNA from blood, that can also be sold to the life science market, the company said.
The company noted that it does not have a sales channel to sell in the life sciences market and is currently searching for a distribution partner.
Sigma Life Science this week released OligoArchitect, an online primer and probe design tool.
This free tool automates design of probes and primers for real-time qPCR assays. It is powered by the Beacon Designer platform from Premier Biosoft, and provides results in real time and supporting templates of up to 10,000 base pairs. It also allows users to adjust various input parameters — such as homopolymer run/repeat maximum langth, G/C clamp length, and maximum primer pair Tm mismatch — to help optimize probe or primer design.
OligoArchitect currently supports SYBR Green I and dual-labeled probe detection chemistries, with additional chemistries and assays expected to be added later this year, SLS said.
The new tool complements Sigma Life Science's portfolio of oligonucleotide and qPCR products and customized oligonucleotide services, the company said.