Agilent Technologies said this week that its SureSelect Target Enrichment System now supports Roche-454 Life Sciences' next-generation sequencing platform.
The SureSelect system enables users to sequence only genomic regions of interest rather than entire genomes, and allows enrichment of targets ranging from smaller than 200 kb to more than 50 Mb in a single tube. The system also enables target enrichment of user-specified regions, the human kinome, the human X chromosome, and the human exome, Agilent said.
Agilent's SureSelect also supports the Illumina Genome Analyzer, Life Technologies' SOLiD 4, and HiSeq sequencing platforms.
Agilent also said this week that Gaithersburg, Md.-based EdgeBio has been appointed as a Certified Service Provider for SureSelect. Agilent said EdgeBio's SureSelect CSP status is specifically applicable to Life Technologies' SOLiD 4 system.
TriLink Biotechnologies this week launched CleanAmp 7-deaza-dGTP, a hot start solution for PCR amplification of problematic GC-rich sequences.
When DNA targets high in GC content are amplified, PCR product formation can often be compromised by inadequate strand separation and the propensity for complex secondary structure formation, TriLink said.
CleanAmp 7-deaza-dGTP is a fusion of the secondary structure-reducing nucleotide analog 7-deaza-dGTP and TriLink's CleanAmp dNTP Hot Start technology. The product is available individually for amplification of routine GC-rich targets or as the CleanAmp 7-deaza-dGTP Mix, which is recommended for more challenging targets with higher GC content, TriLink said.