Amsbio this week introduced the ExpressArt mRNA amplification kit for qRT-PCR and gene expression array studies.
The kits are based on a non-Eberwine method using patented TRinucleotide primers and reagents that minimizes loss of sequence associated with two-round amplification procedures and eliminates 3' bias in amplified products. Amsbio said the kits enable reproducible and precise analysis of challenging samples including formalin-fixed, paraffin-embedded samples, laser-captured microdissections, needle biopsies, and circulating tumor cells.
Epigentek this week introduced the EpiSonic Multi-Functional Bioprocessor 1000, the latest version of its sonication system for high-throughput sample preparation in next-generation ChIP and DNA sequencing.
The EpiSonic 1000 provides optimal DNA fragment size, excellent sample yield and consistency, and enables rapid processing via 96- or 384-well microplates or standard laboratory tubes ranging from 0.2 to 15 mL, Epigentek said.
In addition, the instrument features hands-free and programmed memory operation and can be easily integrated into existing lab workflows and fit to the specifications of all commercial ChIP and sequencing vendors, the company said.
Integrated DNA Technologies said this week that it has developed an extensive quantitative real-time PCR user guide, available for free download on the company's website.
The manual provides user guidance on the entire qPCR process, from RNA isolation to data analysis, IDT said. Further, the manual provides specific information on 5' nuclease assays, including resuspensions and qPCR protocols; and is written to comply with the minimum information for publication of quantitative real-time PCR guidelines.